Differentiation of Protoplasts and of Transformed Plant Cells
H. BINDING and J. REINERT In collaboration with the first authors of this volume This volume is devoted to the development of cell clones and plants from ma nipulated cells: isolated protoplasts, cell fusion bodies, and transformed cells. Isolated protop
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12 Editors W Hennig, Nijmegen and J. Reinert, Berlin
Differentiation of Protoplasts and of Transformed Plant Cells Edited by J. Reinert and H. Binding
With 24 Figures
Springer-Verlag Berlin Heidelberg GmbH
Professor Dr. J. REINERT Institut fiir Pflanzenphysio1ogie Konigin-Luise-StraBe 12-16 D-1000 Berlin 33 Professor Dr. H. BINDING Botanisches Institut der Universităt 01shausenstraBe 40--60 D-2300 Kie1 1
ISBN 978-3-662-21816-7 ISBN 978-3-540-39836-3 (eBook) DOI 10.1007/978-3-540-39836-3
Library of Congress Cataloging in Publication Data. Differentiation of protoplasts and of transformed plant cells. (Results and problems in cell differentiation; 12) Includes bibliographies and index. 1. Plant protoplasts. 2. Plant cell differentiation. 1. Reinert, J. Il. Binding, H. (Horst), 1939- . QK725.D53 1986 581.87'612 86-10218 This work is subject to copyright. Ali rights are reserved, whether the whole or part of the material is concerned, specifically those of translation, reprinting, re-use of illustrations, broadcasting, reproduction by photocopying machine or similar means, and storage in data banks. Under § 54 of the German Copyright Law, where copies are made for other than private use, a fee is payable to the publisher, the amount of the fee to be determined by agreement with the publisher.
© by Springer-Verlag Berlin Heidelberg 1986 Originally published by Springer-Verlag Berlin Heidelberg New York in 1986 Softcover reprint of the hardcover 1st edition 1986 The use of registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. 2131/3130-543210
Contents
Introduction. By H. BINDING and J. REINERT . . . . . . . . . . . .
1
I Isolation and Regeneration of Protoplasts from Higher Plants
By S.C. MAHESHWARI, R. GILL, N. MAHESHWARI, and P. K. GHARYAL (With 1 Figure) 1 Introduction . . . . . . . . . . . . . . . . . . . . . . 2 Isolation of Protoplasts . . . . . . . . . . . . . . . . . 3 Harvesting, Purification, and Culture Methods for Protoplasts . 4 Regeneration of Protoplasts . . . . . . . . . . . . 4.1 Cell Wall Formation. . . . . . . . . . . . . . . 4.2 Cell Division, Colony Formation, and Differentiation 5 Factors Affecting Regeneration of Protoplasts . 5.1 The Chemical Environment . . . . . . . . . 5.1.1 Nutrient Medium and Its Mineral Components 5.1.2 Carbon Source . . . . . . . . . . . . . 5.1.3 Vitamins . . . . . . . . . . . . . . . . 5.1.4 Growth Regulators and Other Supplements . 5.2 The Physical Environment 5.2.1 Density . . 5.2.2 pH . . . . 5.2.3 Temperature 5.2.4 Light . . . 6 General Conclusions, Comments, and Perspectives . References . Addendum . . . . . . . . . . . . . . . . . . . .
3 4 6 7 7 9 12 12 12 14 15 15 17 17 17 18 18 19 25 35
II Protoplasts Fusion and Early Development of Fusants
By H. BINDING, G. KRUMBIEGEL-SCHROEREN, and R. NEHLS (With 20 Figures) 1 2 2.1 2.2
Introduction . . . . . . . . . Natural Plant Protop
