Directed Enzyme Evolution: Advances and Applications
This book focuses on some of the most significant advances in enzyme engineering that have been achieved through directed evolution and hybrid approaches. On the 25th anniversary of the discovery of directed evolution, this volume is a tribute to the pion
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Directed Enzyme Evolution: Advances and Applications
Directed Enzyme Evolution: Advances and Applications
Miguel Alcalde Editor
Directed Enzyme Evolution: Advances and Applications
Editor Miguel Alcalde Department of Biocatalysis Institute of Catalysis and Petrochemistry, CSIC Madrid Spain
ISBN 978-3-319-50411-7 ISBN 978-3-319-50413-1 (eBook) DOI 10.1007/978-3-319-50413-1 Library of Congress Control Number: 2017932555 © Springer International Publishing AG 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Springer imprint is published by Springer Nature The registered company is Springer International Publishing AG The registered company address is: Gewerbestrasse 11, 6330 Cham, Switzerland
Preface
The last couple of decades have witnessed a true revolution in protein engineering that very few could have foreseen, a revolution delivered by the hand of directed evolution. This reliable, robust and effective methodology has enabled us to design enzymes with improved or even novel properties, a mere pipedream only a few years ago. Through consecutive rounds of random mutation, recombination and screening/selection, enzymes tailored a la carte are meeting the needs of different industrial processes, addressing the challenges of working at high temperature or extreme pHs, in non-natural environments or in the presence of strong inhibitors. More significantly, through laboratory evolution we can now harness the catalytic promiscuity of many enzymes to undertake non-natural chemistry for a range of applications that lie within the biotechnology rainbow. Conversely, the rise of directed evolution has demonstrated that the tailoring of enzymes, metabolic pathways or even whole microorganisms is now no longer beyond our reach. Although mimicking the natural process of evolution on a laboratory scale might make us feel ashamed of our immense ignorance regarding protein function (for example through the identification of substitutions of i
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