Distribution of Burkholderia cepacia complex species isolated from industrial processes and contaminated products in Arg
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ORIGINAL ARTICLE
Distribution of Burkholderia cepacia complex species isolated from industrial processes and contaminated products in Argentina A. López De Volder 1,2 & S. Teves 1 & A. Isasmendi 3 & J. L. Pinheiro 3 & L. Ibarra 4 & N. Breglia 1 & T. Herrera 5 & M. Vazquez 4 & C. Hernandez 3 & José Degrossi 2,6 Received: 21 April 2020 / Revised: 21 October 2020 / Accepted: 29 October 2020 # Springer Nature Switzerland AG 2020
Abstract Burkholderia cepacia complex (Bcc) members have clinical relevance as opportunistic pathogens in patients with cystic fibrosis and are responsible of numerous nosocomial infections. These closely related bacteria are also reported as frequent contaminants of industrial products. In this retrospective study, we use PCR and recA gene sequence analysis to identify at species level Bcc isolates recovered from massive consumption products and industrial processes in Argentina during the last 25 years. The sequences obtained were also compared with recA sequences from clinical Bcc isolates deposited in GenBank database. We detected Bcc in purified water and preserved products from pharmaceutics, cosmetics, household cleaning articles, and beverages industries. B. contaminans (which is prevalent among people with cystic fibrosis in Argentina) was the most frequent Bcc species identified (42% of the Bcc isolates studied). B. cepacia (10%), B. cenocepacia (5%), B. vietnamiensis (16%), B. arboris (3%), and the recently defined B. aenigmatica (24%) were also detected. Rec A sequences from all B. cepacia and most B. contaminans industrial isolates obtained in this study displayed 100% identity with recA sequences from isolates infecting Argentinean patients. This information brings evidence for considering industrial massive consumption products as a potential source of Bcc infections. In addition, identification at species level in industrial microbiological laboratories is necessary for a better epidemiological surveillance. Particularly in Argentina, more studies are required in order to reveal the role of these products in the acquisition of B. contaminans infections. Keywords Burkholderia cepacia complex . Contamination . Industrial products . recA PCR
Introduction * José Degrossi [email protected] 1
Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
2
Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
3
Servicio de Microbiología, Hospital de Pediatría Juan P. Garrahan, Buenos Aires, Argentina
4
Servicio de Bacteriología Clínica, Hospital de Niños Ricardo Gutiérrez, Buenos Aires, Argentina
5
Facultad de Ciencias Exactas, Universidad de Buenos Aires, Buenos Aires, Argentina
6
Cátedra de Salud Pública e Higiene Ambiental, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 4° Floor, Ciudad Autónoma de Buenos Aires, Argentina
Burkholderia cepacia complex (Bcc) is a group of at leas
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