DNA barcoding of brown Parmeliae (Parmeliaceae) species: a molecular approach for accurate specimen identification, emph
- PDF / 796,487 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 75 Downloads / 216 Views
ORIGINAL ARTICLE
DNA barcoding of brown Parmeliae (Parmeliaceae) species: a molecular approach for accurate specimen identification, emphasizing species in Greenland Steven D. Leavitt & Theodore L. Esslinger & Eric Steen Hansen & Pradeep K. Divakar & Ana Crespo & Bradley F. Loomis & H. Thorsten Lumbsch Received: 6 March 2013 / Accepted: 6 July 2013 # Gesellschaft für Biologische Systematik 2013
Abstract Warming of Arctic and alpine regions has a substantial impact on high-altitude/-latitude ecosystems. Shifting biomes due to climate change may lead to adjustments in species distributions and potential extinctions. Therefore, detailed monitoring is requisite to assess biologically meaningful shifts in community composition and species distributions. Some Arctic-alpine lichens have been shown to be particularly sensitive to climatic shifts associated with global change. However, accurate identification of lichenized fungal species remains challenging and may limit the effective use of lichens in climate change research. Given the inherent difficulties in accurate identification of lichenized fungi and the potential value of efficient identifications for bio-monitoring research, we investigated the
Electronic supplementary material The online version of this article (doi:10.1007/s13127-013-0147-1) contains supplementary material, which is available to authorized users. S. D. Leavitt : B. F. Loomis : H. T. Lumbsch Department of Botany, The Field Museum, 1400 S. Lake Shore, Drive, Chicago, IL 60605-2496, USA S. D. Leavitt (*) Department of Biology and M. L. Bean Life Science Museum, 193 MLBM, Brigham Young University, Provo, UT 84602, USA e-mail: [email protected] T. L. Esslinger Department of Biological Sciences #2715, North Dakota State University, PO Box 6050, Stevens Hall, Fargo, ND 58108-6050, USA E. S. Hansen Herbarium, Botanical Garden, Natural History Museum of Denmark, University of Copenhagen, Øster Farimagsgade 2 C, 1123 Copenhagen K, Denmark P. K. Divakar : A. Crespo Departamento de Biología Vegetal II, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid 28040, Spain
utility of DNA barcode identification of the 13 brown Parmeliae (Ascomycota) species occurring in Greenland. For these species, we assessed monophyly and genetic distances using the nuclear ribosomal internal transcribed spacer region (ITS), the standard DNA barcode for fungi. We also compared intraspecific distance values to a proposed intra-interspecific threshold value for Parmeliaceae to identify nominal taxa potentially masking previously unrecognized diversity. Our results indicated that the 13 brown Parmeliae species occurring in Greenland can be successfully discriminated using the ITS region. All phenotypically circumscribed species were recovered as well-supported, monophyletic clades. Furthermore, our data supported a barcode gap among congeners for all brown Parmeliae species investigated here. However, high intraspecific genetic distances suggest the potential for previously unrecognized species-lineages in at
Data Loading...
