Identification of some Egyptian leafhopper species (Hemiptera: Cicadellidae) using DNA barcoding
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ORIGINAL ARTICLE
Identification of some Egyptian leafhopper species (Hemiptera: Cicadellidae) using DNA barcoding Azza K. Emam 1 & Heba E. Ibrahim 2 & Ashraf Helmi 1 & Abdoallah Sharaf 3,4 Received: 21 May 2019 / Accepted: 5 November 2019 # Institute of Zoology, Slovak Academy of Sciences 2020
Abstract Leafhoppers (Cicadellidae) occur in all biogeographical regions and feed on a wide range of host plants. Classic identification of leafhoppers is based only on the morphology of male genitalia, while DNA barcoding is an effective tool to identify different leafhopper species in any stage of their life cycle. Phylogenetic relationships among leafhoppers remain unexplored, but recent phylogenies have begun to interpret the evolutionary relationships of previously recognized tribes and subfamilies. Here we used mitochondrial Cytochrome Oxidase I sequences to barcode and determine the phylogenetic relationship among eight leafhopper genera collected from Egypt. Eight leafhopper samples from eight different genera were collected from various plants using a sweeping net and aspirator. Males of seven species were available while females only were available from the one genus (Austroagallia sp.). Novel leafhopper-specific mtDNA COI degenerate primers were designed. Our results showed that the mean of intraspecific COI sequence divergence in leafhoppers was 0.45%, with a range from 0.15% to 1.23%, and the divergences of most species were over 2%. For the first time, we were exploring the evolutionary relationships among surveyed genera (Austroagallia, Empoasca, Eupteryx, Exitianus, Macrosteles, Nephotettix, and Orosius) using a robust phylogenetic supertree. Our results demonstrate that DNA barcoding is likely to be a powerful tool for identifying and understanding leafhoppers’ evolution. Keywords Leafhopper . Mitochondrial COI . DNA barcode . Phylogenetics
Introduction Order Hemiptera comprises over 90,000 species globally (Cranston and Gullan 2009); most of them are economically essential insect groups. They are widely distributed, and many of its members are dangerous pests and vectors of diseases of many economic crops. The effective management of pest
Azza Emam and Abdoallah Sharaf contributed equally to this work. * Abdoallah Sharaf [email protected] 1
Plant Protection Department Faculty of Agriculture, Ain Shams University, Cairo 11241 Egypt
2
Plant Protection Research Institute, Agric. Res. Center, Giza, Egypt
3
Genetic Department, Faculty of Agriculture, Ain Shams University, Cairo, 11241 Egypt
4
Institute of Parasitology, Biology Centre, CAS, Branisovska 31, 370 05 České Budějovice, Czech Republic
species damaging the crop cannot be initiated without precise identification (Sreejith and Sebastian 2014). Cicadellidae is the largest family of Membracoidea with 50 subfamilies and 17,000–20,000 described species (Hodkinson and Casson 1991; Stonedahl and Dolling 1991). Leafhoppers (Cicadellidae) occur in all regions and feed on a wide range of host plants through individual species have often trophically and
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