Downregulation of Cancer-Associated lncRNAs in Peripheral Blood of Multiple Sclerosis Patients
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Downregulation of Cancer-Associated lncRNAs in Peripheral Blood of Multiple Sclerosis Patients Amin Safa 1,2 & Mohammad Taheri 3 & Hamid Fallah 3 & Tayyebali Salmani 3 & Shahram Arsang-Jang 4 & Soudeh Ghafouri-Fard 3 & Mir Davood Omrani 5 Received: 28 May 2020 / Accepted: 19 June 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Recent studies have shown contribution of long non-coding RNAs (lncRNAs) in the pathogenesis of immune-related disorders including multiple sclerosis (MS). Based on the role of these transcripts in the regulation of immune response, peripheral levels of lncRNAs can reflect the level of immune activation. In the present study, we quantified expression of four lncRNAs namely SPRY4-IT1, HOXA-AS2, LINC-ROR, and MEG3 in venous blood of MS patients and controls using quantitative real-time PCR method. Relative expressions of SPRY4-IT1, HOXA-AS2, LINC-ROR, and MEG3 were significantly lower in female MS patients compared with female healthy subjects. For MEG3, this pattern of expression was also observed in male subjects. However, for other lncRNAs, no significant difference was detected between male patients and male controls. Expression of HOXA-AS2 was correlated with progression index (r = 0.36, P < 0.001). Besides, there was a significant correlation between expression of this lncRNA and expression of LINC-ROR in MS patients (r = 0.44, P < 0.0001). There was no other correlation between expression of lncRNAs and clinical data in MS patients. In control group, expressions of none of lncRNAs were correlated with age of persons. Notably, significant correlations were demonstrated between expression levels of all lncRNAs in healthy subjects with r values ranging from 0.23 to 0.42. The current investigation shows dysregulation of lncRNAs in MS patients in a sex-specific manner and warrants further studies to unravel the clinical and therapeutic implications of such dysregulation. Keywords Multiple sclerosis . SPRY4-IT1 . HOXA-AS2 . LINC-ROR and MEG3
Introduction Long non-coding RNAs (lncRNAs) are a category of several transcripts with sizes larger than 200 nucleotides, complex
* Soudeh Ghafouri-Fard [email protected] * Mir Davood Omrani [email protected] 1
Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam
2
Department of Immunology, Ophthalmology and ENT, School of Medicine, Complutense University, Madrid, Spain
3
Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4
Department of Biostatistics and Epidemiology, Cancer Gene Therapy Research Center, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
5
Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
configurations, and versatile functions in diverse biological activities (Zampetaki et al. 2018). As these transcripts are involved in the regulation of gene expression at diverse levels, they have substantial roles in human disorders. Multiple sclero
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