Duplex Detection of Antibiotics in Milk Powder Using Lateral-Flow Assay Based on Surface-Enhanced Raman Spectroscopy
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Duplex Detection of Antibiotics in Milk Powder Using Lateral-Flow Assay Based on Surface-Enhanced Raman Spectroscopy Xiaozhou Li 1,2
&
Xinnuo Wang 3 & Lianyan Wang 4 & Tianyue Yang 1,2 & Deli Wang 2
Received: 10 June 2020 / Accepted: 22 September 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Nowadays, the overuse of antibiotics in cow breeding is a common issue worldwide. Real-time monitoring is critical for antibiotic control of dairy products. Herein, we report a rapid detection method for the duplex detection of two beta-lactam antibiotics (cephalexin and ampicillin) in milk powder using lateral flow (LF) combined with surface-enhanced Raman spectroscopy (SERS). The duplex detection was accomplished using a single test line loaded with two antigens targeting the two corresponding antibody-gold-Raman reporter conjugates. The obtained SERS spectra contributed by the Raman reporter labeled to the two antibodies were analyzed using both peak height method and multiple linear regression (MLR). A good regression fitting was achieved by these two methods (R2 = 0.99 and 0.98, respectively). The limit of detection (LOD) was 10−4 ng/mL for both peak height method and MRL. This study indicated that LF-SERS is a good candidate for real-time multiplex and quantitative analysis for antibiotics in dairy products. Keywords Lateral flow . Immunochromatography . Surface-enhanced Raman spectroscopy . SERS . Antibiotics . Milk
Introduction Overuse of antibiotics in cow breeding is a growing public health concern worldwide (Sachi et al. 2019). Antibiotics residues in dairy products can cause severe health impacts such as antibiotic resistance and are strictly prohibited (Laxminarayan et al. 2013). Among those, beta-lactam antibiotics are widely used for the treatment and prevention of cattle infectious diseases, especially mastitis (Landers et al. 2012; Sachi et al. 2019). Thus, developing a rapid multiplex monitoring
* Xiaozhou Li [email protected] 1
School of Science, Shenyang Ligong University, Shenyang 110159, China
2
School of Environment, Liaoning University, Shenyang 110036, China
3
Liaoning Science and Technology Center for Ecological and Environmental Protection, Department of Ecology and Environment of Liaoning Province, Shenyang 110161, China
4
Department of Computer and Mathematics Teaching, Shenyang Normal University, Shenyang 110034, China
detection method for beta-lactam antibiotics in dairy products is important. Common methods for antibiotic detection are laboratory analytical methods such as high-performance liquid chromatography (HPLC), gas chromatography (GC), mass spectrometry (MS), and the tandem use of these. However, they are highly equipment and technician dependent and have complex and time-consuming procedures (Kenkel 2003). Lateral flow (LF) assay, also named immunochromatography (ICA), is a type of immunoassay accomplished on a strip which utilizes capillary force and immunoadsorption to capture the target antibodies to fulfill the aim of detection. As an i
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