Durotaxis behavior of bEnd.3 cells on soft substrate with patterned platinum nanoparticle array
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ORIGINAL ARTICLE
Durotaxis behavior of bEnd.3 cells on soft substrate with patterned platinum nanoparticle array Xiaomin Wu1,2 · Li Li1,2 · Zecheng Lei1,2 · Fan Yang1,2 · Ri Liu1,2 · Lu Wang1,2 · Xinyao Zhu3 · Zuobin Wang1,2,4 Received: 17 September 2020 / Accepted: 31 October 2020 © King Abdulaziz City for Science and Technology 2020
Abstract The directional arrangement of cells has crucial effect in tissue engineering fields such as wound healing and scar repair. Studies have shown that continuous nanostructures have directional regulatory effect on cells, but whether discontinuous nanostructures have the same regulatory effect on cells is also worthy of further study. Here, a series of discontinuous platinum nanoparticles (PtNPs) patterned on the surface of PDMS (PtNPs-PDMS&Glass) and glass (PtNPs-Glass) substrates were developed to investigate the effect on bEnd.3 cell durotaxis. The laser interference lithography and nanotransfer printing method were employed to fabricate the substrates. It was found that about 80% cells orderly arranged on the PtNPsPDMS&Glass substrate, but only 20% cells orderly arrangement on the PtNPs-Glass substrate, and the number of cells on the PtNPs-PDMS&Glass substrate was five times more than that on the PDMS coated glass substrate (PDMS&Glass). The results suggested that patterning PtNPs on the PDMS substrate not only provided the topographical guidance for cells just like continuous nanostructures, but also promoted cell adhesion and growth. In addition, an improved whole cell coupling model was used to investigate and explain the cell durotaxis from the perspective of mechanism. These findings show the possibility of discontinuous nanostructures in regulating cell arrangement, and offer a useful method for the design of biological functional substrate, as well as help to understand the mechanism of cell durotaxis. Keywords PtNPs · Cell durotaxis · Nanotransfer printing methods · Cell coupling model Abbreviations PDMS Polydimethylsiloxane PtNPs Platinum nanoparticles PDMS&Glass PDMS coated glass PtNPs-PDMS&Glass Platinum nanoparticles patterned on the PDMS coated glass * Li Li [email protected] * Zuobin Wang [email protected] 1
International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China
2
Ministry of Education Key Laboratory for Cross‑Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun 130022, China
3
Key Laboratory of Mechanism Theory and Equipment Design of Ministry of Education, Tianjin University, Tianjin 300350, China
4
JR3CN & IRAC, University of Bedfordshire, Luton LU1 3JU, UK
PtNPs-Glass Platinum nanoparticles patterned on the surface of glass DMEM Dulbecco’s modified Eagle medium FBS Fetal bovine serum PBS Phosphate buffered saline DAPI 4′,6-Diamidino-2-phenylindole PMMA Poly(methyl methacrylate) SEM Scanning electron microscopy AFM Atomic force microscope FM Fluorescence microscopy ECM Extracellular matrix bE
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