Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred
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ORIGINAL RESEARCH PAPER
Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor Alfredo Eduardo Maiorano . Elda Sabino da Silva . Rafael Firmani Perna . Cristiane Ange´lica Ottoni . Rosane Aparecida Moniz Piccoli . Rube´n Cuervo Fernandez . Beatriz Guilarte Maresma . Maria Filomena de Andrade Rodrigues
Received: 14 May 2020 / Accepted: 14 September 2020 Ó Springer Nature B.V. 2020
Abstract Objective Fructooligosaccharides (FOS) are prebiotic substances that have been extensively incorporated in different products of food industry mostly for their bifidogenic properties and economic value. The main commercial FOS production comes from the biotransformation of sucrose and intracellular and extracellular microbial enzymes–fructosyltransferases (FTase). Aspergillus oryzae IPT-301 produces FTase. In order to increase its production, this study focuses
on evaluating the effects of different agitation speed and aeration rates which affect yields in a stirred tank bioreactor. Results Agitation had more influence on cell growth than aeration. The maximum intracellular FTase activity and the volumetric productivity of total intracellular FTase were obtained at 800 rpm and 0.75 vvm, and reached values of 2100 U g-1 and 667 U dm-3 h-1, respectively. The agitation speed had a strong influence on the activity of extracellular FTase
A. E. Maiorano (&) E. S. da Silva R. A. M. Piccoli M. F. de Andrade Rodrigues Bionanomanufacturing Center, Institute for Technological Research (IPT), Av. Prof. Almeida Prado 532, Sa˜o Paulo, Sa˜o Paulo 05508-901, Brazil e-mail: [email protected]
R. C. Fernandez B. G. Maresma Instituto Cubano de Investigaciones de los Derivados de la Can˜a de Azu´car (ICIDCA), Vı´a Blanca 804, Carretera Central, San Miguel del Padro´n, 11000 Ciudad de la Habana, Cuba
R. F. Perna Institute of Science and Technology, Federal University of Alfenas (ICT/UNIFAL-MG), Jose´ Aure´lio Vilela Road, 11999 BR 267 Km 533, Poc¸os de Caldas, Minas Gerais 37715-400, Brazil C. A. Ottoni Biosciences Institute, Sa˜o Paulo State University (UNESP), Prac¸a Infante Dom Henrique s/no, Sa˜o Vicente, Sa˜o Paulo 11330-900, Brazil C. A. Ottoni Instituto de Estudos Avanc¸ados do Mar (IEAMar), Sa˜o Paulo State University, Av. Joa˜o Frascisco Bensdorp 1178, Sa˜o Vicente, Sa˜o Paulo 11350-011, Brazil
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produced which reached the maximum amount of 53 U cm-3. The higher value of total activity obtained was 22,831 U dm-3 at 0.75 vvm and 800 rpm. Conclusion Aeration rates and agitation speed showed strong influence upon the growth and production of fructosyltransferase from Aspergillus oryzae IPT-301 in media containing sucrose as carbon source. The control of aeration rate and agitation speed can be a valuable fermentation strategy to improve enzyme production. Keywords Fructosyltransferase bfructofuranosidase Fructooligosaccharides Aspergillus Enzyme production KLa
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