Effect of Albumin on Tumor Cells In Vitro
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min on Tumor Cells In Vitro
V. G. Arzumanyan1, M. V. Kiselevskii2, I. M. Ozhovan1, and O. A. Svitich1 Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 8, pp. 227-230, August, 2020 Original article submitted May 8, 2020 The study examined the effect of BSA on cultured cells of breast cancer, erythroleukemia, and ovarian carcinoma. BSA cytotoxic activity was examined with light microscopy and spectrophotometry in the concentration range of 6.25 to 50 mg/ml. The high concentrations of BSA disrupted the tumor cells in parallel with formation of vesicular debris. The destruction parameters were similar in diverse types of cells: BSA (50 mg/ml) disrupted 75.5±0.7% breast cancer cells, 75.8±0.2% erythroleukemia cells, and 78.8±0.1% cells of ovarian carcinoma (p≥0.05). The effect of BSA was dose-dependent in each type of cells. The data attest that BSA can disrupt various tumor cells in vitro. Key Words: albumin; tumor cells; breast cancer; erythroleukemia; ovarian carcinoma
Serum albumin is a unique plasma protein involved in the maintenance of plasma oncotic pressure, binding and transport of low-molecular substances and exogenous ligands, redox reactions, maintenance of acid-base balance in the blood and adequate capillary permeability, and control of apoptosis and homeostasis. Moreover, albumin is also implicated in plastic function; it affects vascular integrity and exerts immunomodulating effects due to binding to various products and metabolites of microorganisms [1,7]. We have previously demonstrated that albumin in concentrations similar to those in blood serum can lyse the cellular walls and membranes of conventionally pathogenic microorganisms [4] and even disrupt spermatozoa [2]. This work was designed to examine the effects of albumin on tumor cells in vitro.
MATERIALS AND METHODS The study used BSA solutions (Serva) and cell cultures of human erythroleukemia (K562), ovarian carcinoma (SCOV-3), and breast cancer (SCBR-3). The cells were cultured in complete medium RPMI-1640 I. I. Mechnikov Research Institute of Vaccines and Sera; 2N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia. Address for correspondence: [email protected]. V. G. Arzumanyan
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(PanEco) containing 4 mM L-glutamine, 1% penicillin/streptomycin, and 10% fetal calf serum. Incubation was carried out in 50-ml plastic culture flasks containing in 10 ml medium and 106 cell/ml. The tumor cell suspension was cultured in a CO2 incubator at 37°C and 5% CO2 for 48 h until the formation of cell monolayer. The cytotoxic activity of BSA against tumor cells was assessed according to previously described modified method based on determination of integrity of cytoplasmic membrane of eukaryotes [3]. The cells of adherent cultures SCOV-3 and SCBR-3 were carefully detached from the plastic surface with a scrapper; the cells of suspension culture K562 were transferred into plastic tubes. The cells were suspended in 1.5 ml physiological saline and centrifuged at 10
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