Effect of baffle structure on flow field characteristics of orbitally shaken bioreactor
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RESEARCH PAPER
Effect of baffle structure on flow field characteristics of orbitally shaken bioreactor Zhiming Lu1 · Chengtuo Li1 · Liangqi Fei1 · Hongliang Zhang1 · Yuhui Pan1 Received: 18 June 2020 / Accepted: 22 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Disposable orbitally shaken bioreactors have been widely used for mammalian cell culture in suspension. Three kinds of baffle structures: vertical baffle, inclined baffle and horizontal baffle were designed in this work. The flow fields of the shaking bioreactor with different baffle structures were simulated, and the turbulence, dissolved oxygen and shear strain rate of the bioreactor were analyzed. The results showed that the quasi-steady-state flow patterns of the unbaffled shaking bioreactors were broken for the bioreactors with the strengthening effects of baffles. The mixing and the oxygen volumetric mass transfer coefficient (kLa) (simulated results) were improved significantly, and the shear strain rates were also increased greatly for the baffle bioreactors. The shear strain rates of the baffle bioreactors were mainly in the range of 0–20 s−1, and they were still low enough for CHO cell cultures. Keywords Baffled bioreactor · CFD simulation · Flow field · Oxygen transfer · Shear environment
Introduction As the key equipment in cell cultures, bioreactors have been studied by more and more scholars and experts in recent years [1, 2]. Jia et al. [3] studied the oxygen transfer method in a shaking bioreactor. Zhu et al. [4] analyzed the effects of filling volume and shaking speed on kLa, energy dissipation rate and shear stress in 600-mL orbitally shaken bioreactor. Lu et al. [5] found that turbulence parameters increase with shaking frequency, while decrease with filling volume proportionally in shaking bioreactor. Compared with the traditional agitated bioreactors, the shaking bioreactor has the advantages of low shear strain rates, easy operation and low energy loss. In particular, the advantage of low shear strain rate is very suitable for cell cultures without cell walls [6, 7]. At the same time, they also have certain shortcomings, for example, when the volume of the shaking bioreactor becomes larger, the oxygen transfer rate in the bioreactor will be decreased obviously, which is not good for cell growth [8, 9].
* Zhiming Lu [email protected] 1
College of Mechanical Engineering, Zhejiang University of Technology, Hangzhou, China
To improve the mixing and dissolved oxygen performance of the shaking bioreactor, many researches on the flow characteristics of the bioreactor have been carried out. Bai et al. [10] designed a conical bottom and studied the effect of the bottom structure on the dissolved oxygen performance of the shaking bioreactor. The results showed that there was larger free surface area for the cone-bottom bioreactor than for the flat-bottom one, and it was good to improve the dissolved oxygen performance. Hang et al. [11] built the CFD models of the shaking bioreactor with cone bottom an
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