Effect of composition and method of preparation of 2-hydroxyethyl methacrylate/gelatin hydrogels on biological in vitro
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ORIGINAL PAPER
Effect of composition and method of preparation of 2-hydroxyethyl methacrylate/gelatin hydrogels on biological in vitro (cell line) and in vivo (zebrafish) properties Simonida Lj. Tomić 1 & Marija M. Babić 1 & Jovana S. Vuković 1 & Lidija Djokić 2 & Aleksandar Pavić 2 & Jasmina Nikodinovic-Runić 2 Received: 25 December 2019 / Accepted: 17 July 2020 / Published online: 22 September 2020 # The Polymer Society, Taipei 2020
Abstract We have studied the effect of compositions and methods of preparation on the physico-chemical and biocompatible behavior of the hydrogel matrices. Hydrogel matrices are synthesized by free radical polymerization of 2-hydroxyethyl methacrylate net and with gelatin. Highly porous hydrogel structures were obtained by porogenation, and by cryogenic treatment followed by freezedrying. All samples were characterized for structural, morphological, absorption, degradation and in vitro (healthy human fibroblast cell line) and in vivo (zebrafish Danio rerio) biocompatible properties. The obtained results show that cryo samples, especially with gelatin show better, favorable absorption, morphological and biocompatible properties in comparison with PHEMA samples, which makes these materials highly attractive for biomedical uses. Keywords 2-Hydroxyethyl methacrylate . Gelatin . Hydrogel . Morphology . Absorption . Biocompatible in vitro and in vivo properties
Introduction Hydrogels based on natural or synthetic polymeric components are considered suitable for biomedical applications. Hydrogel (aquagel) is a network of polymeric chains, threedimensional fluid (water) swollen structures. They are composed mainly of hydrophilic homopolymers or copolymers. They swell but are insoluble in water. Insolubility is due to chemical or physical cross-links which also provide shape. Hydrogels are super permeable and closely conjure up as natural living tissue due to their favorable water content, absorbance, and soft texture. The smart properties of hydrogel
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10965-020-02219-w) contains supplementary material, which is available to authorized users. * Simonida Lj. Tomić [email protected] 1
Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade 11 000, Serbia
2
Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, Belgrade, Serbia
coupled with high versatility and high tunability of material properties lead to innate research. Polymeric hydrogels have proved to be one of the most efficient matrices for repairing and regenerating a wide variety of tissues or organs. Hydrogel applications range from basic usage as lubricants, contact lenses, and burn healing agents to higher performances such as controlled drug delivery systems and specialized carriers for various macromolecules [1–7]. Natural polymers offer diverse advantageous properties such as inherent biocompatibility, biodegradability, and biologically recognizable m
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