Effect of EMD on the orthodontically induced root resorption repair process in rats
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ORIGINAL ARTICLE
Effect of EMD on the orthodontically induced root resorption repair process in rats Qin Hu1,2 · Jianping Zhou1,2 · Xiaolin Xu3 · Hongwei Dai1,4 Received: 6 October 2017 / Accepted: 13 November 2017 © Springer Medizin Verlag GmbH, ein Teil von Springer Nature 2018
Abstract Background While different levels of root resorption may occur in orthodontic treatment, several preventive approaches have been reported. Nevertheless, little is known about the effect of enamel matrix derivative (EMD) on root repair during orthodontic tooth movement. Objective To evaluate the effect of EMD on root resorption repair following the application of orthodontic force. Materials and methods A force of 100 g was exerted for 14 days on the left maxillary first molars of twenty 10-week-old Sprague-Dawley rats divided into the EMD and control groups (n = 10 per group). In the EMD group, repeatedly injection of Emdogain® was administered after the appliance was removed, while phosphate-buffered saline was administered in the control group. In vivo microcomputed tomography (CT), haematoxylin and eosin (H&E) staining, and immunohistochemistry were then used to evaluate the effect of EMD on the process of root repair. Results In the EMD group, the observed decrease in root resorption crater volume and increase in both the bone volume fraction and trabecular thickness were significantly greater than those in the control group. H&E staining showed that the periodontal fibres were relatively regular in arrangement and that the surface of the cementum was smooth in the EMD group. Immunohistochemical analysis showed higher bone morphogenetic protein 2 (BMP-2) and bone sialoprotein (BSP) expression levels in the EMD group than in the control group. In addition, the osteoprotegerin (OPG) and receptor activator of NF-κB ligand (RANKL) expression levels were similar in both groups. Conclusion EMD enhanced the repair process following orthodontically induced root resorption in rats.
Keywords Enamel matrix derivative · Orthodontic tooth movement · Root repair · Animal model · Micro-CT
Qin Hu and Jianping Zhou contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00056-017-0119-8) contains supplementary material, which is available to authorized users. Hongwei Dai
[email protected] 1
Department of Orthodontics, College of Stomatology, Chongqing Medical University, Chongqing, China
2
Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China
3
Department of Dentistry, College of Stomatology, Chongqing Medical University, Chongqing, China
4
Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China
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Q. Hu et al.
Auswirkung eines Schmelzmatrixproteins auf reparative Prozesse nach orthodontisch induzierter Wurzelresorption im Rattenmodell Zusammenfassung Hintergrund Während einer kieferorthopädischen Behandlung kann es zu Wurzelresorptionen unterschiedlichen Ausmaßes kommen, wobei vers
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