Effect of herb-partitioned moxibustion in improving tight junctions of intestinal epithelium in Crohn disease mediated b
- PDF / 1,632,208 Bytes
- 11 Pages / 595.22 x 842 pts (A4) Page_size
- 106 Downloads / 160 Views
Special Topic for 973 Program
Effect of herb-partitioned moxibustion in improving tight junctions of intestinal epithelium in Crohn disease mediated by TNF-α-NF-κB-MLCK pathway 隔药灸对 TNF-α-NF-κB-MLCK 介导克罗恩病肠上皮细胞紧密连接的影响 Gao Yan-ling (高艳玲)1, Wang Yu-ning (王雨宁)1, Guo Ya-jing (郭娅静)1, Sun Yi (孙怡)1, Wang Yi-ran (王逸然)1, Zhou Jing (周竞)2, Zhao Ji-meng (赵继梦)1,3, Wu Huan-gan (吴焕淦)1,3, Shi Yin (施茵)1,3 1 Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China 2 Shanghai TCM-integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200082, China 3 Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China
Abstract Objective: To explore the effect of herb-partitioned moxibustion (HPM) on tight junctions (TJs) of intestinal epithelial cells in Crohn disease (CD) mediated by tumor necrosis factor-α (TNF-α)-nuclear factor kappa B (NF-κB)-myosin-lightchain kinase (MLCK) pathway. Methods: Forty-eight male Sprague-Dawley rats were randomly divided into a normal control (NC) group, a model control (MC) group, an HPM group and a mesalazine (MESA) group, with 12 rats in each group. Trinitrobenzene sulfonic acid (TNBS) was administered to establish CD models. When the model was confirmed a success, the HPM group rats were treated with HPM at Tianshu (ST 25) and Qihai (CV 6), while the MESA group rats were given MESA solution by lavage. When the intervention finished, the colonic epithelial tissues were separated, purified and cultured in each group to establish the intestinal epithelial barrier model in vitro, and TNF-α was added (100 ng/mL) in the culture medium and maintained for 24 h to establish an increased epithelial permeability model. Transepithelial electrical resistance (TEER) was used to examine the permeability of the barrier; Western blot was used to observe the expressions of the proteins related to TJs of intestinal epithelial cells mediated by TNF-α-NF-κB-MLCK pathway; immunofluorescence staining was used to observe the expressions and distributions of tight junction proteins in the intestinal epithelium. Results: After TNF-α induction, compared with the MC+TNF-α group, the TEER value increased significantly in the HPM+TNF-α and MESA+TNF-α groups (both P
Data Loading...
