Effect of Phloroglucinol on in Vitro Rooting of Sugarcane ( Saccharum spp. cv C90-469)
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Effect of Phloroglucinol on in Vitro Rooting of Sugarcane (Saccharum spp. cv C90-469) Rafael Go´mez-Kosky1 • Pablo Machado Armas1 • Midiala Bermu´dez Calimano1 • Aydiloide Bernal Villegas1 ´ lvarez Ferreiro1 • Dion D. Daniels2 • Laisyn Posada Pe´rez3 Yaimi Otero1 • Dunia Nu´n˜ez Jaramillo1 • Jercy A
•
Received: 13 February 2020 / Accepted: 4 October 2020 Ó Society for Sugar Research & Promotion 2020
Abstract The objective of this study was to determine the effect of phloroglucinol on in vitro rooting of sugarcane cultivar C90-469. A completely randomized experimental design was used with seven treatments, three concentrations of phloroglucinol (10, 15 and 20 mg L-1) in combination or not with 1.3 mg L-1 of indole-3-acetic acid (IAA) and as control the liquid culture medium without phloroglucinol. After 15 days of culture, the number of shoots rooted, height, number of leaves, fresh weight of the in vitro plant (gFW), number of roots, length of the main root and the total chlorophyll content (SPAD units) were evaluated. In the ex vitro acclimatization phase, the survival of the in vitro plants was evaluated 15 days after transplanting. Concentration of 20 mg L-1 of phloroglucinol combined with 1.3 mg L-1 of IAA and 40 g L-1 of sucrose had 100% rooting. A greater number of roots per plant, the formation of root hairs and a high survival percentage (87.8%) of the in vitro plants under ex vitro acclimatization conditions in a shade house were recorded. Keywords Organogenesis Sugarcane In vitro rooting Phloroglucinol Ex vitro acclimatization
& Rafael Go´mez-Kosky [email protected]; [email protected] 1
Estacio´n Territorial de Investigaciones de La Can˜a de Azu´car (ETICA) Centro Villa Clara, Instituto de Investigaciones de La Can˜a de Azu´car (INICA), Autopista Nacional km 246, CP 53100 Villa Clara, Ranchuelo, Cuba
2
Faculty of Science & Technology, University of Belize, Hummingbird Avenue, Belmopan, Belize
3
Instituto de Biotecnologı´a de Las Plantas, Universidad Central ‘‘Marta Abreu’’ de Las Villas., Carretera a Camajuanı´ km 5 , CP 54830 Villa Clara, Santa Clara, Cuba
Phloroglucinol (1,3,5-trihydroxybenzene or phloroglucin) is a product of the degradation of phloridzin and precursor of the lignin biosynthesis pathway. It is a phenolic compound not used much for its properties as a plant growth promoter (Teixeira da Silva et al. 2013). However, it has various applications in the medical and pharmaceutical industries (Crockett et al. 2008; Saddique et al. 2010; Stein et al. 2012). In vitro culture showed a positive effect on lignification (Ross and Castillo 2010), in the reduction of hyperhydricity (Ross and Grasso 2010) and in the formation of calluses at the base of shoots in woody species (Teixeira da Silva et al. 2013). In plants cultured in vitro, phloroglucinol increased root formation (Bopana and Saxena 2009; Agud et al. 2010; Teixeira da Silva et al. 2013; Posada-Pe´rez et al. 2016). In vitro rooting is known as the transfer stage of the aerial parts (shoo
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