Effect of silver and copper nanoparticles on adsorption and fluorescence of tryptophan on the surface of bactericidal te
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ORIGINAL ARTICLE
Effect of silver and copper nanoparticles on adsorption and fluorescence of tryptophan on the surface of bactericidal textile I. S. Petrik1 · A. M. Eremenko1 · A. P. Naumenko2 · A. V. Rudenko3 Received: 4 December 2018 / Accepted: 20 February 2020 © King Abdulaziz City for Science and Technology 2020
Abstract Biomedical preparations based on cotton tissue modified with nanoparticles of silver and copper with pronounced bactericidal properties have been obtained. The bactericidal activity of Ag- and Ag/Cu-modified cotton fabrics (assessed by the inhibition zone of bacterial growth) was evaluated against bacteria E. coli, K. pneumonia, P. aeruginos, S. aureus, E. faecalis. To suppress a toxic effect of nanoparticles we investigate the possibilities of adsorption modification of cotton fabrics contained Ag and Ag/Cu nanoparticles by amino acid tryptophan (Trp) and the effect of metals on Trp fluorescence spectra. Adsorption isotherms of Trp demonstrate a significantly higher adsorption capacity of Ag/Cu/cotton compared to Ag/cotton. The fluorescence of Trp is quenched on the modified tissue surfaces due to the formation of complexes with silver and copper ions, released from the metal nanoparticles. It is shown that the complexation of copper and silver nanoparticles with Trp on the surface of tissues does not reduce their bactericidal action against a number of microorganisms. Keywords Silver · Copper · Tryptophan · Adsorption · Fluorescence quenching · Bactericidal cotton
Introduction Cotton tissue modified with nanoparticles (NPs) of silver and copper with pronounced bactericidal properties has been obtained by gentle heat treatment of metal ions on the surface of the fabrics (Eremenko et al. 2016). Mechanism of the NPs formation is not entirely clear. Cellulose contains reducing oligosaccharides. Their aldehyde function presumably can promote the process of silver and copper ions reduction. We can assume also the concentration of metal ions around OH-groups of cellulose (Eremenko et al. 2016). The water molecules are included in the coordination sphere of metal ions, simultaneously forming a hydrogen bond with OH-groups of cellulose. We have previously shown the release of silver and copper ions in water from the textile modified by nanoparticles (NPs) (Eremenko et al. 2018). It * A. M. Eremenko [email protected] 1
Chuiko Institute of Surface Chemistry of NAS of Ukraine, 17, General Naumov Street, Kyiv 03164, Ukraine
2
Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Street, Kyiv 01601, Ukraine
3
Institute of Urology of Acad. of Med. Sci of Ukraine, Yu Kotsyubynskogo Street, 9‑A, Kyiv 04053, Ukraine
was found out that during the three consecutive 24-h soaking cycles in water, approximately 56% of the Ag ions are overall released from the Ag/cotton fabrics (Ag-cf), as well as 14% Ag+ and 17% Cu2+ from the Ag/Cu/cotton fabrics (Ag/Cu-cf). Besides, the bactericidal activity of tissues after leaching of metal ions from the surface of nanoparticles remained high, wh
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