In vitro evaluation of bactericidal effect of silver and gold-silver nanoparticles coated with silicon dioxide on Xantho

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In vitro evaluation of bactericidal effect of silver and gold-silver nanoparticles coated with silicon dioxide on Xanthomonas fragariae Juana-Cristina Ibarra-Arán1, Douglas Rodríguez-Martínez2, Eugenio Rodríguez-González1, Jesús-Roberto González-Castillo1 1 Instituto Politécnico Nacional – Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada Unidad Altamira, Km. 14.5 Carretera Tampico-Puerto Industrial Altamira, Altamira, Tamaulipas, México 2 Crop Protection – Department of Applied Research, Driscoll´s, Ave. Moctezuma 144, Piso 1, Ciudad del Sol, Zapopan, Jalisco, Z.C. 45050, México ABSTRACT In this work it was evaluated the bactericidal effect of silver and silver-gold nanoparticles coated with silicon dioxide on Xanthomonas fragariae. Nanoparticles were synthesized by combining laser ablation and chemical synthesis techniques. Irradiating the samples at the same wavelength where nanoparticles exhibit its maximum absorbance is possible to photo induce the bactericidal effect by detonating the surface plasmon resonance. The results showed that the minimum bactericidal concentration (MBC) of silver nanoparticleVZDVȝ0 (referred to [AgNO 3 ]) and for silver-gold nanoparticles ZHUHȝ0 UHIHUUHGWR>$J12 3 -HAuCl 4 ]) at 60 minutes of irradiation.

INTRODUCTION Strawberry (Fragaria x ananassa) is an important crop in Mexico. In fact, Mexico is the third largest producer of strawberry, after the United States and China [1]. In this country, this crop generates 1.1% of the generated value by the agricultural sector, besides being an important export product [2]. The strawberry angular leaf spot (ALS), caused by Xanthomonas fragariae was first reported in Minnesota in 1962 and has since been found in several U.S. states as well as strawberry-growing regions in Europe, South America, Africa, Australia, and New Zealand becoming a restrictive factor for production of both fruit and nursery plants to worldwide level [3]. This bacterium was reported for first time in Michoacán, Mexico by Fernandez-Pavía et. al. [4]. The main economic importance for some markets nurseries around the world, is that X. fragariae is listed as A2 quarantined pest by the European and Mediterranean Plant Protection Organization (EPPO) [5], being necessary that commercial nurseries must certify plants pathogen free if producer countries wish to export to Europe [6]. Treatments with coppercontaining products have some effectiveness, but they have to be applied very intensively, with a risk of phytotoxicity [7]. Since no effective chemical control is available, currently there is a continuing search for new alternatives for ALS control in plants [8]. Among the most promising is the use of nanoparticles of metals such as silver and gold. Historically, compounds such as silver nitrate and silver sulfadiazine were used as antiseptics and bactericides in burns, respectively [9]. In the case of agricultural field applications, there have been used titanium- silver dioxide nanoparticles

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