Effect of Substrate Surface Modification on Biomineralization of Osteoblasts
- PDF / 389,996 Bytes
- 6 Pages / 612 x 792 pts (letter) Page_size
- 119 Downloads / 235 Views
0950-D10-09
Effect of Substrate Surface Modification on Biomineralization of Osteoblasts Yizhi Meng1, Xiaolan Ba2, Seo-Young Kwak3, Elaine DiMasi3, Meghan Ruppel3, Lisa Miller3, Shouren Ge2, Nadine Pernodet2, Miriam Rafailovich2, and Yi-Xian Qin1 1 Biomedical Engineering, Stony Brook University, Psychology A, 3rd Floor, Stony Brook, NY, 11794-2580 2 Materials Science & Engineering, Stony Brook University, Stony Brook, NY, 11794 3 National Synchrotron Light Source, Brookhaven National Laboratory, Upton, NY, 11973
ABSTRACT Understanding how biomineralization occurs in the extracellular matrix (ECM) of bone cells is crucial to the development of a successfully engineered bone tissue scaffold, and to date there has not been a well-established method for the quantitative examination of bone mineralization in situ. We investigated the mechanical properties of MC3T3-E1 osteoblast-like cells and the crystalline properties of their biomineralized ECM in vitro using shear modulation force microscopy (SMFM), confocal laser scanning microscopy (CLSM), synchrotron X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). The elastic modulus of the mineralizing cells increased at time points corresponding to mineral production, whereas that of the non-mineralizing cells did not vary significantly over time. CLSM showed a restructuring of the F-actin fiber network of mineralizing cells with time, which indicates remodeling activities in the cytoskeleton and was not seen in the non-mineralizing cells. Both XRD and FTIR showed that the mineralizing subclone produced hydroxyapatite in situ and that the non-mineralizing subclone was in fact weakly biomineralizing. INTRODUCTION More than 1.8 million people over the age of 65 were treated in emergency departments for fall-related injuries in 2003, and more than 421,000 were hospitalized (1). Between 20% and 30% of fall victims suffer hip fractures or head traumas and are vastly debilitated due to reduced mobility and independence, and are at high risk for premature death (1). Hip fracture is also the leading cause of death among fall-related injuries and greatly reduces the quality of life for its victims (1). It is estimated that by the year 2040, the number of hip fractures will exceed 500,000 (1). Every year, more than 300,000 hip fractures result from osteoporosis, and 50% of women as well as 25% of men over 50 years old will have an osteoporosis-related fracture in their lifetime (4). The cost of treating and caring for patients with osteoporosis was 18 billion dollars in 2002 and is expected to rise. There has been increasing interest in designing biocompatible materials for the treatment of bone fractures, with the notable use of both primary and cloned cells as seeding material (3). However, a systematic, quantifiable way of testing for the osteogenic capabilities of cultured bone cells in situ is still lacking. Moreover, traditional methods of testing for the formation of bone mineral in osteoblasts are difficult to quantify and may introduce false positive
Data Loading...
