Effective isolation of antioxidant Phelligridin LA from the fermentation broth of Inonotus baumii by macroporous resin
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RESEARCH PAPER
Effective isolation of antioxidant Phelligridin LA from the fermentation broth of Inonotus baumii by macroporous resin Tianwen Wang1 · Shiwei Sun3 · Chen Liang1 · Hui Li3 · Ao Liu3 · Hu Zhu2 Received: 2 May 2020 / Accepted: 23 June 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Phelligridin LA (PLA) is a natural product with vigorous free radical scavenging activities accumulated in the liquid fermentation of herbal medicinal fungus Inonotus baumii. Aiming to establish an efficient isolation method of PLA from the fermentation broth, we evaluated the adsorption of PLA by macroporous resins. The best resin ADS-17 was screened for six candidates with various physical properties and adsorption behaviors. Studies on the thermodynamics and kinetics of the process revealed that the adsorption reaction could take place spontaneously, which implied that the heat generated in adsorption might compensate for the decrease in entropy. The Freundlich theory could be utilized to fit the experimental data. The pseudo-second-order equation could describe the process, and the adsorption rate was primarily controlled by liquid film diffusion and pore diffusion. The influencing operation factors (temperature, pH, and the ratio of fermentation broth to resin) of the adsorption process were optimized with response surface methodology. The optimized condition (temperature 22.81 °C, pH 5.19, and the ratio of fermentation broth to resin or RLS 5.11) supported an adsorption rate of 97.03%. These findings would be indispensable for further optimization of the efficient separation of PLA from the fermentation broth, and the fermentation production of PLA in which separation would be included. Keywords Phelligridin LA · Macroporous resin · Bioseparation optimization · Response surface methodology · Thermodynamics and kinetics · Diffusion
Introduction The medicinal fungus Inonotus baumii has been utilized in the recipe of herbal medicine for quite a long history, especially in the region of East Asia [1–4]. According to Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00449-020-02398-2) contains supplementary material, which is available to authorized users. * Hu Zhu [email protected] 1
College of Life Sciences, and Institute for Conservation and Utilization of Agro‑Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, China
2
Fujian Provincial University Engineering Research Center of Industrial Biocatalysis, College of Chemistry and Materials Science, Fujian Normal University, 32 Shangsan Road, Fuzhou 350007, People’s Republic of China
3
Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266580, China
traditional knowledge, the most valuable part of the fungus is the fruit body directly gleaned from its native growing site [5, 6]. This intuitional preference severely limits the extensive utilization of I. baumii, because the fruit body
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