Isolation and characterization of potent bioactive fraction with antioxidant and UV absorbing activity from Aloe barbade
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Isolation and characterization of potent bioactive fraction with antioxidant and UV absorbing activity from Aloe barbadensis Miller gel Anirban Ray & S. Dutta Gupta & Sampad Ghosh
Received: 20 April 2012 / Accepted: 24 November 2012 # Society for Plant Biochemistry and Biotechnology 2012
Abstract The methanolic extract of Aloe vera L. gel was subjected to antioxidant guided fractionation with silica gel column chromatography to screen the potent fraction. The antioxidant capacities of different fractions were evaluated using DPPH radical scavenging assay and correlated with total phenol content. Total phenolic contents of different fractions were determined according to the Folin-Ciocalteu spectrophotometric method. The positive correlation was observed between DPPH radical scavenging assay and total phenolic contents indicating that phenolics in Aloe vera L. gel were fundamental contributor of antioxidant activity. Third pooled fraction was identified as potential fraction with highest antioxidant potential. This fraction indicated the presence of well resolved fluorescent components. Characteristic UV–vis absorption and HPLC analysis indicates that aloin take part in antioxidant potential attributed to aloe gel. Keywords Aloe vera . Antioxidant-activity . UV absorption . HPLC . Aloin Abbreviations FTIR Fourier transform infrared spectroscopy UV Ultra violet HPLC High performance liquid chromatography Electronic supplementary material The online version of this article (doi:10.1007/s13562-012-0178-2) contains supplementary material, which is available to authorized users. A. Ray (*) : S. Dutta Gupta Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, West Bengal 721302, India e-mail: [email protected] S. Ghosh Department of Chemistry, National Institute of Technology, Jamshedpur, Jharkhand 831014, India
RP LSD DPPH
Reverse phase Least significant difference Diphenyl picrylhydrazyl
Aloe vera L. (Aloe barbadensis Miller) belongs to liliaceae family (presently known as Asphodelaceae, APG III system of 2009) that possesses therapeutic and antioxidant properties. Gels from Aloe vera leaves are widely used in skin care products and as health supplement. The major components of aloe may be divided in five groups (Choi and Chung 2003) e.g. Phenolics (anthraquinones, flavonoids), saccharides (mannose, glucomannan, acemannan etc.), vitamins (B1, B2 etc.), enzymes (amylase, carboxypeptidase etc.) and low molecular weight substances (cholesterol, salicylic acid etc). The anthraquinones in Aloe vera mainly composed of aloe emodin, aloin, aloetic acid, anthranol, barbaloin, isobarbaloin, emodin, ester of cinnamic acid (Hamman 2008). Free radicals interact with cellular constituents such as lipids and deoxyribonucleic acid, perpetuating cellular damages (Rice-Evans et al. 1996). Free radicals can also damage the genetic materials, create aging and wrinkles and dismay the general resistance mechanism, increasing the risk of melanoma (Kumar et al. 2009). Oxyge
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