Effectiveness of Platelet-Rich Plasma in the Prevention of Chlamydia-Induced Hydrosalpinx in a Murine Model
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REPRODUCTIVE ENDOCRINOLOGY: ORIGINAL ARTICLE
Effectiveness of Platelet-Rich Plasma in the Prevention of Chlamydia-Induced Hydrosalpinx in a Murine Model Sheena M. Rippentrop 1 & Zhi Huo 2 & Zengzi Zhou 2 & Francisco Zaldana 3 & Stephanie Hernandez 1 & Randal D. Robinson 1 & Guangming Zhong 2 Received: 15 May 2020 / Accepted: 20 September 2020 # Society for Reproductive Investigation 2020
Abstract Chlamydia trachomatis (C. trachomatis) is a major pathogen implicated in the formation of hydrosalpinx in the female reproductive tract. In mice, a related strain of Chlamydia, Chlamydia trachomatis (C. trachomatis) can induce almost 100% bilateral hydrosalpinx. This model was used as a hydrosalpinx induction model to test whether oviduct delivery of platelet-rich plasma (PRP) can attenuate chlamydia induction of hydrosalpinx in a mouse model. Mice were infected intravaginally with Chlamydia muridarum organisms, and 21 days after the infection, PRP was instilled into the lumen of one oviduct, and a sham instillation with phosphate buffer solution was performed on the contralateral oviduct. Mice were then sacrificed at designated time points after infection for oviduct pathologic evaluation including incidence, severity, and histopathologic grade of chronic inflammation. Oviduct instillation of PRP was associated with a 36% reduction in the incidence of hydrosalpinx and a 33% reduction in severity compared with sham. The median grade of chronic inflammation on histopathology was significantly lower with PRP instillation compared with sham and control. No differences were observed in vaginal or rectal shedding of C. muridarum between the test group and the control group. In short, the results suggest that oviduct instillation of PRP can significantly reduce the incidence and severity of C. muridarum-induced hydrosalpinx without affecting chlamydial infection courses in CBA/J mice. Keywords Platelet-rich plasma . PRP . Hydrosalpinx
Introduction
Capsule Platelet rich plasma significantly reduces chlamydia induced hydrosalpinx in a murine model and may serve as a novel therapy in prevention and treatment of chlamydia-induced pathology. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s43032-020-00329-w) contains supplementary material, which is available to authorized users. * Sheena M. Rippentrop [email protected] 1
Present address: Department of OB-GYN, University of Texas Health Science Center San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA
2
Department of Microbiology, University of Texas Health Science Center San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA
3
Department of Pathology, University of Texas Health Science Center San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA
Chlamydia trachomatis (C. trachomatis) is an obligate intracellular gram-negative bacterium that causes the most common sexually transmitted bacterial infection. Those at highest risk for chlamydia include younger women with multiple sexual partners [1–4
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