Efficient degradation of ivermectin by newly isolated Aeromonas taiwanensis ZJB-18,044
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ORIGINAL PAPER
Efficient degradation of ivermectin by newly isolated Aeromonas taiwanensis ZJB-18,044 Yuanshan Wang . Meihua Gong . Xianlin Wang . Xiaolun Peng . Yuwei Wang . Jiahui Guan . Dongyuan Cheng . Chunyue Weng . Yuguo Zheng
Received: 23 December 2019 / Accepted: 5 September 2020 Ó Springer Nature B.V. 2020
Abstract Ivermectin (IVM) is a widely used antiparasitic agent and acaricide. Despite its high efficiency against nematodes and arthropods, IVM may pose a threat to the environment due to its ecotoxcity. In this study, degradation of IVM by a newly isolated bacterium Aeromonas taiwanensis ZJB-18,044 was investigated. Strain ZJB-18,044 can completely degrade 50 mg/L IVM in 5 d with a biodegradation ability of 0.42 mg/L/h. Meanwhile, it exhibited high tolerance (50 mg/L) to doramectin, emamectin, Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10532-020-09909-8) contains supplementary material, which is available to authorized users. Y. Wang M. Gong X. Wang X. Peng Y. Wang J. Guan D. Cheng C. Weng Y. Zheng (&) Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No. 18, Chaowang Road, Hangzhou 310014, Zhejiang, People’s Republic of China e-mail: [email protected] Y. Wang e-mail: [email protected] M. Gong e-mail: [email protected] X. Wang e-mail: [email protected]
rifampicin, and spiramycin. It can also efficiently degrade doramectin, emamectin, and spiramycin. The IVM degradation of strain ZJB-18,044 can be inhibited by erythromycin, azithromycin, spiramycin or rifampicin. However, supplement of carbonyl cyanide m-chlorophenylhydrazone, an uncoupler of oxidative phosphorylation, can partially recover the IVM degradation. Moreover, strain ZJB-18,044 cells can pump out excess IVM to maintain a low intracellular IVM concentration. Therefore, the IVM tolerance of strain ZJB-18,044 may be due to the regulation of the intracellular IVM concentration by the activated macrolide efflux pump(s). With the high IVM degradation efficiency, A. taiwanensis ZJB-18,044 may Y. Wang e-mail: [email protected] J. Guan e-mail: [email protected] D. Cheng e-mail: [email protected] C. Weng e-mail: [email protected] Y. Wang M. Gong X. Wang X. Peng Y. Wang J. Guan D. Cheng C. Weng Y. Zheng Engineering Research Center of Bioconversion and Biopurification, Ministry of Education, Zhejiang University of Technology, No. 18, Chaowang Road, Hangzhou 310014, Zhejiang, People’s Republic of China
X. Peng e-mail: [email protected]
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Biodegradation
serve as a bioremediation agent for IVM and other macrolides in the environment. Keywords Aeromonas taiwanensis Biodegradation Efflux pump Ivermectin Tolerance Abbreviations IVM Ivermectin AVM Avermectin SARSSevere acute respiratory syndrome CoV-2 coronavirus 2 CCCP Carbonyl cyanide mchlorophenylhydrazone MSM Mineral salts medium LB Luria–Bertani medium medium M
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