Patterns of Lignocellulosic Sugar Assimilation and Lipid Production by Newly Isolated Yeast Strains From Chilean Valdivi
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Patterns of Lignocellulosic Sugar Assimilation and Lipid Production by Newly Isolated Yeast Strains From Chilean Valdivian Forest Gabriela Valdés 1,2,3 & Regis Teixeira Mendonça 2,3 & Carolina Parra 2 & George Aggelis 1 Received: 18 May 2020 / Accepted: 16 July 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
Three yeast strains were isolated from decaying wood of Chilean Valdivian forest and identified as Meyerozyma guilliermondii, Scheffersomyces coipomensis, and Sugiyamaella paludigena. These strains were able to efficiently grow on the major monomers contained in Pinus spp. and Eucalyptus spp. wood that includes glucose (Glc), xylose (Xyl), and mannose (Man), showing at 28 °C higher uptake rates for Man, and in some cases for Glc, than for Xyl, used as single carbon sources. Nevertheless, in cultures performed on sugar mixtures, the strains displayed a notable preference for Glc. Additionally, in sugar mixtures, the absence of regulatory mechanisms in sugar assimilation (e.g., catabolic repression) was observed and documented when the activities of several enzymes involved in sugar assimilation (i.e., phosphoglucose isomerase, phosphomannose isomerase, and xylulokinase) were determined. The activity of the key enzymes involved in the onset of lipid accumulation (i.e., NAD+-ICDH) and in fatty acid (FA) biosynthesis (i.e., ATP:CL) indicated a significant accumulation of storage lipids (i.e., up to 24%, w/w) containing oleic and palmitic acids as the major components. The present paper is the first report on the potential of M. guilliermondii, S. coipomensis, and S. paludigena as oleaginous yeasts. We conclude that the new isolates, being able to simultaneously assimilate the major lignocellulosic sugars and efficiently convert them into oily biomass, present a biotechnological potential which deserve further investigation. Keywords Meyerozyma guilliermondii . Scheffersomyces coipomensis . Sugiyamaella paludigena . Lignocellulosic sugars . Single cell oil
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12010-02003398-4) contains supplementary material, which is available to authorized users.
* George Aggelis [email protected] Extended author information available on the last page of the article
Applied Biochemistry and Biotechnology
Abbreviations ATP:CL FA Glc Man NAD+-ICDH PGI PMI PUFA SCO TCA XK Xyl
ATP-citrate lyase Fatty acid Glucose Mannose NAD+-dependent isocitrate dehydrogenase Phosphoglucose isomerase Phosphomannose isomerase Polyunsaturated fatty acid Single cell oil Tricarboxylic acid Xylulokinase Xylose
Introduction Among oleaginous microorganisms, yeasts are the most likely candidates for lipid (single cell oil, SCO) production with interesting biotechnological applications. SCO derived from yeasts usually contains saturated and monosaturated fatty acids (FAs) of C16 and C18 families and some polyunsaturated fatty acids (PUFAs) with a restricted number of double bonds, such as linoleic acid (C18:2, n−6) a
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