EhEm1 , a novel Em -like protein from Eutrema halophilum , confers tolerance to salt and drought stresses in rice
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EhEm1, a novel Em-like protein from Eutrema halophilum, confers tolerance to salt and drought stresses in rice Dianjun Xiang & Lili Man
Received: 25 February 2017 / Accepted: 31 October 2017 / Published online: 20 January 2018 # Springer Science+Business Media B.V., part of Springer Nature 2018
Research highlights 1. EhEm1 was expressed in response to various stresses. 2. The EhEm1 protein could protect LDH activity. 3. EhEm1-overexpressing rice enhanced tolerance to salt and drought stresses with less yield loss. 4. Overexpression of EhEm1 up-regulated the transcript levels of OsCDPKs and rab16a.
in young seedlings. The EhEm1 protein could prevent the lactate dehydrogenase (LDH) enzyme from inactivation caused by salt and desiccation treatments, and contribute to the stabilization of LDH activity. The sensitivity of transgenic rice seeds to exogenous ABA showed that EhEm1 functioned in an ABA-dependent signal pathway in response to abiotic stresses. Compared with wild type (WT) plants, EhEm1-overexpressing rice enhanced tolerance to salt and drought stresses based on better germination performances, higher survival rates, reduced chlorophyll damage, more accumulation of proline, less accumulation of malondialdehyde, and increased peroxidase activity under salt and drought stresses. The EhEm1-overexpressing rice showed less yield loss than did WT rice under salt and drought stresses. In addition, overexpression of the EhEm1 gene in rice could up-regulate transcript levels of several stress-responsive genes, including OsCDPK6, OsCDPK9, OsCDPK13, and rab16a under both salt and drought conditions, indicating that enhanced salt and drought tolerance of transgenic rice could be involved in the upregulation of OsCDPK genes. Thus, this work provides an insight into the tolerance of plants to abiotic stresses.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11032-017-0750-5) contains supplementary material, which is available to authorized users.
Keywords LEA protein . EhEm1 . Abiotic stress tolerance . Eutrema halophilum . Rice
D. Xiang College of Agriculture, Inner Mongolia University for Nationalities, Tongliao 028042, China
Introduction
L. Man (*) College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028042, China e-mail: [email protected]
Environmental stress factors, such as high salinity, drought, extreme temperature, and heavy metals, are frequently confronted by higher plants. Abiotic stresses
Abstract Late embryogenesis abundant (LEA) proteins play active and significant roles in stress resistance of plants. In this work, a novel Em-like gene, EhEm1, was isolated and characterized from salt cress. Bioinformatic analysis revealed that the EhEm1 protein contained three 20-mer conserved motifs (N-motif, M-motif, and C-motif), shared high homology with Em proteins from other species, and was categorized into group 1 LEA proteins. The EhEm1 was expressed only in the roots during the seedling period, and its transcript levels were sign
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