The Nicotiana tabacum L. major latex protein-like protein 423 ( NtMLP423 ) positively regulates drought tolerance by ABA
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RESEARCH ARTICLE
Open Access
The Nicotiana tabacum L. major latex protein-like protein 423 (NtMLP423) positively regulates drought tolerance by ABA-dependent pathway Heng Liu1, Xiaocen Ma1, Shaohua Liu1, Bingyang Du1, Nini Cheng2, Yong Wang1 and Yuanhu Zhang1*
Abstract Background: Drought stress is an environmental factor that limits plant growth and reproduction. Little research has been conducted to investigate the MLP gene in tobacco. Here, NtMLP423 was isolated and identified, and its role in drought stress was studied. Results: Overexpression of NtMLP423 improved tolerance to drought stress in tobacco, as determined by physiological analyses of water loss efficiency, reactive oxygen species levels, malondialdehyde content, and levels of osmotic regulatory substances. Overexpression of NtMLP423 in transgenic plants led to greater sensitivity to abscisic acid (ABA)-mediated seed germination and ABA-induced stomatal closure. NtMLP423 also regulated drought tolerance by increasing the levels of ABA under conditions of drought stress. Our study showed that the transcription level of ABA synthetic genes also increased. Overexpression of NtMLP423 reduced membrane damage and ROS accumulation and increased the expression of stress-related genes under drought stress. We also found that NtWRKY71 regulated the transcription of NtMLP423 to improve drought tolerance. Conclusions: Our results indicated that NtMLP423-overexpressing increased drought tolerance in tobacco via the ABA pathway. Keywords: NtMLP423, Drought stress, ABA, Nicotiana tabacum
Background Drought stress is not conducive to plant growth and development, as it can cause changes in plant morphology and damage to cells [1, 2]. Plants have evolved many complex physiological and biochemical mechanisms to adapt to drought. The plant hormone, abscisic acid, regulates the physiological processes of plants under biotic and abiotic stresses [3].
* Correspondence: [email protected] 1 State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, P.R. China Full list of author information is available at the end of the article
Abscisic acid is a key sesquiterpene which is participated in many important processes of plant growth and development, and controls many genes related to stress adaptation responses and osmotic adjustment [4–6]. The increase in ABA synthesis under drought stress can promote stomatal closure and reduce transpiration loss [7]. Due to the role of ABA in response to drought stress, genes involved in the biosynthesis of ABA have been identified, such as 9-cis-epoxycarotenoid dioxygenase (NCED), xanthoxin dehydrogenase/reductase (ABA2), and ABA-aldehyde oxidase 3 (AAO3) [8, 9]. In Arabidopsis, NCED3 contributes to ABA accumulation in response to drought stress [10], while the aba2 and
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