Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid
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C
hinese Journal of Integrative Medicine
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Available online at link.springer.com/journal/11655 Journal homepage: www.cjim.cn/zxyjhen/zxyjhen/ch/index.aspx E-mail: [email protected]
Acupuncture Research
Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid System WANG Ke1,2, YONG Yue3, ZHOU Jia1, ZHOU Wen-xiong1, GUO Jun4, and CHEN Tong-yu1 Objective:: To investigate the action mechanisms of electroacupuncture (EA) on postoperative ABSTRACT Objective Methods:: Male C57BL/6 mice (5–7 weeks old) were randomly divided into: the sham immunosuppression. Methods injury group, the surgical trauma stressed group, the EA group [surgery + 2/100 Hz EA at Neiguan (PC 6)], and the EA+ Nal (surgery + EA + intraperitoneal injection of naloxone). Abdominal surgical trauma stress mice model was established. EA was performed on bilateral PC 6 acupoints by an EA apparatus (2/100 Hz) for 20 min once a day for 3 days. The mRNA expressions of MOR, DOR, and KOR in thymus and L3–L5 dorsal root ganglions (DRG) were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and the protein expressions of MOR, DOR, and KOR in thymus were measured by Western blot. Flow cytometry Results:: Surgical trauma assay was used to detect the levels of T lymphocyte subtypes in the peripheral blood. Results induced decreased the mRNA expression level of MOR in both thymus (P
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