Electrochemical immunoassay for the carcinoembryonic antigen based on Au NPs modified zeolitic imidazolate framework and
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ORIGINAL PAPER
Electrochemical immunoassay for the carcinoembryonic antigen based on Au NPs modified zeolitic imidazolate framework and ordered mesoporous carbon Yingcong Zhang 1 & Ze Zhang 1 & Shengzhong Rong 1,2 & Hongwei Yu 1 & Hongmin Gao 3 & Ping Ding 4 & Dong Chang 1 & Hongzhi Pan 5 Received: 1 November 2019 / Accepted: 24 March 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract An electrochemical immunoassay for the carcinoembryonic antigen is described. It is based on the use of Au NPs modified zeolitic imidazolate framework (ZIF-8) and ordered mesoporous carbon (OMC). Au NPs@ZIF-8 was synthesized by reduction of chloroauric acid. It serves as immobilization support nanocarrier to increase antibody loading due to its large surface area. OMC was dropped on a glassy carbon electrode to improve electrochemical signals due to enhanced electrical conductivity. Differential pulse voltammetry was carried out to record electrochemical responses (best measured at 0.26 V vs. Ag/AgCl). The immunosensor demonstrated excellent electrochemical performance with a linear determination range of 5 pg mL−1 to 400 ng mL−1 and a determination limit of 1.3 pg mL−1 (S/N = 3). The sensor also exhibited high selectivity, good stability, and acceptable reproducibility.
Keywords CEA . Immunosensor . ZIF-8 . Gold nanoparticles
Introduction
Yingcong Zhang and Ze Zhang contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04235-5) contains supplementary material, which is available to authorized users. * Dong Chang [email protected] * Hongzhi Pan [email protected] 1
Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University, Shanghai 200000, People’s Republic of China
2
Public Health School, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang, People’s Republic of China
3
School of Public Health, Wuhan University of Science and Technology, Wuhan, HuBei Province 430065, People’s Republic of China
4
Xiang Ya School of Public Health, Central South University, Changsha 410078, People’s Republic of China
5
Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai 201399, People’s Republic of China
Carcinoembryonic antigen (CEA) is a tumor marker normally expressed in limited areas of human body. Overexpression of CEA contributes to improving the diagnosis of many diseases, including colorectal cancer [1], lung cancer [2], breast cancer [3], pancreatic cancer [4], and many other benign tumors [5]. Various immunological methods have been exploited for CEA determination, including electrochemical immunoassay [6, 7], photoelectrochemical immunoassay [8], fluoroimmunoassay [9], chemiluminescence immunoassay [10, 11], plasmonic immunosensor [12], and so on. Electrochemical immunoassay is identified as the predominant analytical determination technology for clinical diagnosis due to its advantages such as cost effectiveness, extremely high sensitivity, fast r
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