Electrochemical sensor using carbon nanotube composites for chronic-degenerative diseases diagnosis
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MRS Advances © 2020 Materials Research Society DOI: 10.1557/adv.2020.253
Electrochemical sensor using carbon nanotube composites for chronicdegenerative diseases diagnosis Antonio Rowland Ramos-Díaz1; Ramon Gómez Aguilar2; Hugo MartínezGutiérrez3; Jaime Ortiz-López4 1
Instituto Politécnico Nacional, ESIQIE, Av. Instituto Politécnico Nacional s/n, 07738 Ciudad de México, México.
2
Instituto Politécnico Nacional, UPIITA, Av. Instituto Politécnico Nacional, 07340 Ciudad de México, México.
3
Instituto Politécnico Nacional, CNMN, Av. Luis Enrique Erro s/n, 07738 Ciudad de México, México.
4
Instituto Politécnico Nacional, ESFM, Av. Instituto Politécnico Nacional, 07738 Ciudad de México, México.
Abstract
Sensitive and selective detection for cancer biomarkers is critical in cancer clinical diagnostics. In this work, we report an electrochemical detection platform for the carbohydrate antigen tumor marker 15-3 (CA15-3). It is based on a composite material of poly [2-methoxy-5- (2-ethylhexyloxy) -1,4-phenylenevinylene] (MEH-PPV) and multiwalled carbon nanotubes (MWCNT), which deposited as active layer of an organic thinfilm transistor. This layer was surface functionalized with the Anti-CA15-3 monoclonal antibody. The devices have a favorable electrical output response for VSD source-drain potentials between 0 to 5 volts, and VG as 8 volts. Once the antigen (CA15-3) is 1
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recognized by the antibody, the electrical response is diminished. The test has a linear response in the concentration range of 0–30 U mL - 1 of CA 15–3, with a lower detection limit of ~1 U mL - 1 and a stability of 90% with respect to the initial values after storing the device for two weeks. The method was successfully applied to the determination of CA15-3 in serum samples. Possibly, this used composite material has a greater scope and can be applied to another type of detection scheme.
INTRODUCTION: The early diagnosis of chronic-degenerative diseases is one of the main objectives of research groups around the world [1-3]. Hence the growing interest in diagnostic and prognostic biomarkers that can be identified in body fluids [4,5]. Although many commercial kits have been applied in clinical immunoassays for biomarkers detection, conventional methods always have some disadvantages as time-consuming, expensive or labor-intensive. Thus, it is needed to develop a highly sensitive, less expensive, and fast detection methods as alternatives to the conventional immunoassay procedures. Nowadays, methods are being developed for the detection of biomarkers as proteins, antigens or enzymes using electrochemical sensors, that with just a minimal or no sample preparation make real-time monitoring of biomarkers possible due to use of functional nanomaterials that improve electrochemical activity and biocompatibility
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