Electroporation Protocols Microorganism, Mammalian System, and Nanod
This third edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted microorganism, mammalian single cells, tissues, and animals for prevention and treatment of disease. It builds on the success of
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Shulin Li · Lingqian Chang Justin Teissie Editors
Electroporation Protocols Microorganism, Mammalian System, and Nanodevice Third Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, UK
For further volumes: http://www.springer.com/series/7651
For over 35 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-bystep fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice. These hallmark features were introduced by series editor Dr. John Walker and constitute the key ingredient in each and every volume of the Methods in Molecular Biology series. Tested and trusted, comprehensive and reliable, all protocols from the series are indexed in PubMed.
Electroporation Protocols Microorganism, Mammalian System, and Nanodevice Third Edition
Edited by
Shulin Li MD Anderson Cancer Center, The University of Texas, Houston, TX, USA
Lingqian Chang School of Biological Science and Medical Engineering, Beihang University, Beijing, China Institute of Nanotechnology for Single Cell Analysis (INSCA), Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing, China
Justin Teissie Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, Toulouse, France
Editors Shulin Li MD Anderson Cancer Center The University of Texas Houston, TX, USA Justin Teissie Institut de Pharmacologie et de Biologie Structurale Universite´ de Toulouse, CNRS, UPS Toulouse, France
Lingqian Chang School of Biological Science and Medical Engineering Beihang University Beijing, China Institute of Nanotechnology for Single Cell Analysis (INSCA), Beijing Advanced Innovation Center for Biomedical Engineering Beihang University Beijing, China
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9739-8 ISBN 978-1-4939-9740-4 (eBook) https://doi.org/10.1007/978-1-4939-9740-4 © Springer Science+Business Media, LLC, part of Springer Nature 2020 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered name
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