Mammalian Chromosome Engineering Methods and Protocols
The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with the successful production of transgenic animals with engineered chromosomes and chromosomes developed for pharmaceutical prote
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Mammalian Chromosome Engineering Methods and Protocols
Edited by
Gyula Hadlaczky Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary
Editor Gyula Hadlaczky, Ph.D. Institute of Genetics Biological Research Center Hungarian Academy of Sciences Szeged, Hungary [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-098-0 e-ISBN 978-1-61779-099-7 DOI 10.1007/978-1-61779-099-7 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011923965 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality. Transgenic animals with engineered chromosomes have been produced with success, and chromosomes developed for pharmaceutical protein production are now ready for the medical industry. Engineered chromosomes have also been used in preclinical model experiments for ex vivo stem-cell therapy. This volume is intended to provide the reader with up-to-date information on this rapidly evolving field, and will attempt to take the reader into the exciting realm of chromosomal engineering from the basic principles to the practical applications of these new technologies. The five overview and ten protocol chapters cover the engineering of chromosomes with extrachromosomal vectors and transposon systems, the manipulation of naturally occurred minichromosomes, the generation and engineering of synthetic artificial chromosomes, and the induced de novo platform artificial chromosome system. The efforts of the aut
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