Electrospun tubes based on PLA, gelatin and genipin in different arrangements for blood vessel tissue engineering
- PDF / 1,787,648 Bytes
- 19 Pages / 439.37 x 666.142 pts Page_size
- 23 Downloads / 191 Views
Electrospun tubes based on PLA, gelatin and genipin in different arrangements for blood vessel tissue engineering Abraham Alejandro Leyva‑Verduzco1 · María Mónica Castillo‑Ortega1 · Lerma Hanaiy Chan‑Chan2 · Erika Silva‑Campa3 · Ramsés Galaz‑Méndez4 · Ricardo Vera‑Graziano5 · José Carmelo Encinas‑Encinas1 · Teresa Del Castillo‑Castro1 · Dora Evelia Rodríguez‑Félix1 · Hisila del Carmen Santacruz‑Ortega1 · Irela Santos‑Sauceda6 Received: 19 June 2019 / Revised: 4 October 2019 / Accepted: 8 December 2019 © Springer-Verlag GmbH Germany, part of Springer Nature 2019
Abstract Four tubular membranes based on PLA and gelatin with different arrangements (PLA, gelatin, PLA-gelatin Blend and PLA/gelatin Core/Shell) were prepared by the electrospinning technique, the gelatin within the materials was crosslinked with genipin and all the meshes were characterized by SEM, ATR-FTIR, TGA, DSC, XRD and mechanical tests, also a viability essay and a confocal microscopy of HUVECs in contact with the materials were carried out. A 50% decrement of cellular viability was observed in the fibers with Core/Shell structure since the first day of culture compared with the tissue culture polystyrene, due to solubilization of noncrosslinked gelatin. Keywords Electrospinning · Poly(lactic acid) · Gelatin · Tissue engineering
* María Mónica Castillo‑Ortega [email protected] 1
Departamento de Investigación en Polímeros y Materiales, Universidad de Sonora, 83000 Hermosillo Sonora, Mexico
2
Departamento de Física, Universidad de Sonora, 83000 Hermosillo Sonora, Mexico
3
Departamento de Investigación en Física, Universidad de Sonora, 83000 Hermosillo Sonora, Mexico
4
GSE Biomedical, 83150 Hermosillo Sonora, Mexico
5
Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico
6
Centro de Investigación y de Estudios Avanzados Unidad Querétaro, 76230 Santiago de Querétaro, Querétaro, Mexico
13
Vol.:(0123456789)
Polymer Bulletin
Introduction In order to solve the problems that generate some of the cardiovascular diseases, an invasive procedure known as artery bypass is used. In this intervention, the flow of blood is reflected using a vascular graft from a healthy place to another place where the blood is not nourishing the tissue due to an occlusion derived from an inflammatory process. For example, in the coronary artery bypass surgery, an autograft is commonly used to support the coronary artery system with blood from the aorta artery. The graft is usually the saphenous vein, the radial artery or the internal thoracic artery of the same patient [1, 2]. This involves, inconveniently, the necessity of another surgical intervention in order to extract the autograft [3]. To avoid a second surgical intervention, the autograft is replaced with a tube made of a biocompatible plastic material as Dacron and expanded Teflon. These materials have been used over the years, but when they are applied as small diameter blood vessel grafts (
Data Loading...
