Elucidation of sequence polymorphism in fuzzless-seed cotton lines
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ORIGINAL ARTICLE
Elucidation of sequence polymorphism in fuzzless‑seed cotton lines Marina Naoumkina1 · Gregory N. Thyssen1,2 · David D. Fang1 · Ping Li1 · Christopher B. Florane1 Received: 10 April 2020 / Accepted: 10 October 2020 © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020
Abstract Most commercially produced cotton cultivars have two types of fibers on the seed coat, short fuzz and long lint. Lint fiber is used in the textile industry, while fuzz is considered an undesirable trait. Both types of fibers are believed to be controlled by the same regulators; however, their mechanisms of actions are still obscure. Cotton fiber mutants provide an excellent system to study the genes that regulate fiber development. Here we described four uncharacterized and three previously reported cotton mutants with fuzzless seed phenotypes. To evaluate whether or not the genes previously associated with fuzzless seed phenotypes have mutations we sequenced whole genomic DNA of seven mutants and wild type varieties. We identified multiple polymorphic changes among the tested genes. Non-synonymous SNPs in the coding region of the MML3A gene was common in the six mutant lines tested in this study, showing both dominant and recessive fuzzless phenotypes. We have mapped the locus of the causative mutation for one of the uncharacterized fuzzless lines using an F2 population that originated from a cross between the dominant fuzzless mutant and a wild type. Further, we have clarified the current knowledge about the causative n2 mutations by analyzing the sequence data and previously reported mapping data. The key genes and possible mechanisms of fiber differentiation are discussed in this study. Keywords Gossypium hirsutum · Cotton fiber · Fuzzless · Fiberless · Single nucleotide polymorphism
Introduction Cotton fibers are single-celled seed trichomes derived from the outer epidermis of the ovule. There are two types of seed trichomes observed in cultivated cotton species, long lint fibers that are easily detached from the epidermis, and fuzz fibers that are short fibers strongly adhere to the seed coat (Applequist et al. 2001). Lint and fuzz fibers show developmental differences: lint initiation starts on the day or before Communicated by Stefan Hohmann. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00438-020-01736-z) contains supplementary material, which is available to authorized users. * Marina Naoumkina [email protected] 1
Cotton Fiber Bioscience Research Unit, United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Southern Regional Research Center (SRRC), 1100 Robert E. Lee Blvd, New Orleans, LA 70124, USA
Cotton Chemistry and Utilization Research Unit, USDA-ARS-SRRC, 1100 Robert E. Lee Blvd, New Orleans, LA 70124, USA
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anthesis while fuzz initiates a few days later; lint fibers grow to lengths of approximately 30 mm, whereas fuzz fibers, for reaso
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