Employment of hordein subunit polymorphisms in establishing selection criteria for high quality malting barley ( Hordeum
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J. Crop Sci. Biotech. 2010 (June) 13 (2) : 91 ~ 97 DOI No. 10.1007/s12892-010-0057-0 RESEARCH ARTICLE
Employment of Hordein Subunit Polymorphisms in Establishing Selection Criteria for High Quality Malting Barley (Hordeum vulgare L.) Yong Jin Lee1, Tong Geon Lee2, Woong Bae Jeon1, Dae Yeon Kim1, Min Jeong Hong1, Man Bo Lee1, Jong Nae Hyun3, Mi Jung Kim3, Mi Ja Lee2, Cheol Su Park3, Yong Weon Seo1* Division of Biotechnology, Korea University, Anam-Dong, Seongbuk-Gu, Seoul 136-713, Korea Institute of Life Science and Natural Resources, Korea University, Anam-Dong, Seongbuk-Gu, Seoul 136-713, Korea 3 National Institute of Crop Science, RDA, Gyeonggi-do 441-857. Korea 1
2
Received: May 18, 2010 / Revised: May 23, 2010 / Accepted: June 11, 2010 Ⓒ Korean Society of Crop Science and Springer 2010
Abstract Cereal seed storage proteins are encoded by complex multigene families and their subunit profiles are highly related to end-use qualities. Each fraction of albumin and hordein was extracted and its subunit profile was evaluated in related to malt and grain quality parameters. The purpose of this study was to provide selection criteria for high quality malting barley using grain and malt quality parameters and biochemical-genetic information. Grain and malt quality of 13 local adaptability test (LAT) lines were evaluated for malting process. A total of 16 germplasm accessions of high or low seed storage protein content were also evaluated for biochemicalgenetic analysis. The correlation coefficients among quality parameters were analyzed. Several important quality parameters in brewing process showed significant positive or negative correlations. Seed storage protein subunits of albumin and hordein of all tested lines and accessions were evaluated using 12% 1D SDS-PAGE. Scored data of protein subunit's presence or absence was applied to Agglomerative Hierarchical Clustering (AHC) for statistical analysis and showed specific grouping patterns among tested lines. Clustered lines with subunit information were highly related with agricultural performance and grain and malt qualities. Based on the profiles of seed storage protein subunits, association of hordein subunit of 38, 43, and 65 kDa with high malt scored lines was found. The obtained results would provide improved selection criteria for high quality malting barley in the malting barley breeding program. Key words: malting barley, parameter, protein subunits, quality
Introduction Barley is one of the major crop species which is widely used for human consumption and animal feeding as well as in the production of malt. In the brewing process, barley is the raw material for malting and subsequently, the production of beer (Celus et al. 2006). A number of quality parameters to predict malting quality were proposed by American Society of Brewing Chemists Yong Weon Seo ( ) Tel: +82-2-3290-3005 Fax: +82-2-3290-3501 E-mail: [email protected]
The Korean Society of Crop Science
(ASBC) and European Brewery Convention (EBC) (American Society of Brewing Chemists 1989; Europe
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