Identification of AFLP markers associated with spot blotch resistance through single marker analysis in barley ( Hordeum
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ORIGINAL PAPER
Identification of AFLP markers associated with spot blotch resistance through single marker analysis in barley (Hordeum vulgare L.) M. I. E. Arabi1 · A. AL. daoude1 · L. Mokrani2 · A. Shoaib1 · M. Jawhar1 Received: 16 June 2020 / Accepted: 6 November 2020 © Akadémiai Kiadó Zrt. 2020
Abstract Spot blotch (SB) caused by the fungus Cochliobolus sativus, is an economically important disease on barley worldwide. Molecular analysis of SB resistance using quantitative trait locus (QTL) analysis can improve the efficiency of the breeding process. In the current work, and to accelerate development of SB-resistant barley cultivars, AFLP markers linked to SB resistance have been identified by using F2 recombinant inbred lines derived from a cross between the resistant barley cv. Banteng and the susceptible cv. WI2291. Single marker analysis (SMA) based on t-test revealed that out of 82 AFLP markers; five AFLP markers AAC × CTG (700 bp), ACT × CAT (600 bp), ACT × CAT (400 bp), ACT × CTG (550 bp) and AGG × CAG (490 bp) were identified. The data showed that SMA-based t-test could be a useful analysis for dominant markers such AFLP in breeding programs, and that the AFLP detected markers can be employed in genomics-assisted breeding for selection of SB-resistant barley genotypes. Keywords Barley · cochliobolus sativus · AFLP marker · SMA · QTL analysis
Introduction Barley (Hordeum vulgare L.) is a grass species (Poaceae), and one of the oldest cultivated crops grown in temperate climates worldwide. In Syria, barley is the major rainfed crop and is the main animal feed in the country; however, changing farming systems by replacement of traditional barley rotations as well as the replacement of traditional cultivars may have a large influence on plant diseases (Van Leur et al. 1989). Spot blotch caused by the fungal pathogen Cochliobolus sativus [asexual stage: Bipolaris sorokiniana (Sacc. in Sorok.) Shoem.] has become a serious problem on cultivation of barley, wheat and other cereals in different parts of the world, such as South and North America (Mathre 1997), Morocco ( Rehman et al. 2020), Syria (Arabi and Jawhar Communicated by A. Börner. * M. I. E. Arabi [email protected] 1
Department of Molecular Biology and Biotechnology, AECS, P. O. Box 6091, Damascus, Syria
Faculty of Science, Damascus University, P. O. Box 86, Damascus, Syria
2
2003) and several Asian countries (Knudsen et al. 1995; Sharma and Duveiller 2006). It causes significant yield losses as well as quality of barley grain (Clark et al. 1979; Kumar et al. 2002). Application of fungicides can be used to control SB but the use of resistant cultivars is still considered the most effective and environmentally safe appropriate solution (Kavita et al. 2017; Duveiller and Sharma 2009). However, in Syria, sources of complete resistance to SB have not been detected, and current barley genotypes are considered to be only moderately resistant against SB disease (Arabi and Jawhar 2004). Barley breeding to develop SB-resistant cultivars based
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