Engraftment of bioengineered three-dimensional scaffold from human amniotic membrane-derived extracellular matrix accele
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ORIGINAL PAPER
Engraftment of bioengineered three‑dimensional scaffold from human amniotic membrane‑derived extracellular matrix accelerates ischemic diabetic wound healing Davood Nasiry1 · Ali Reza Khalatbary2 · Mohammad‑Amin Abdollahifar1 · Abdollah Amini1 · Mohammad Bayat1 · Afshin Noori3 · Abbas Piryaei1,3,4 Received: 16 April 2020 / Revised: 7 July 2020 / Accepted: 3 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Human amniotic membrane (HAM) is traditionally used for the treatment of non-healing wounds. However, high density of HAM-matrix (HAM-M) diminishes cellular contribution for successful tissue regeneration. Herein we investigated whether a bioengineered micro-porous three-dimensional (3D) HAM-scaffold (HAM-S) could promote healing in ischemic wounds in diabetic type 1 rat. HAM-S was prepared from freshly decellularized HAM. Then, 30 days after inducing diabetes, an ischemic circular excision was generated on rats’ skin. The diabetic animals were randomly divided into untreated (Diabetic group), engrafted with HAM-M (D-HAM-M group) and HAM-S (D-HAM-S group). Also, non-diabeticuntreated rats (Healthy group) were considered as control. Stereological, molecular, and tensiometrical assessments were performed on post-surgical days 7, 14, and 21. We found that the volumes of new epidermis and dermis, the numerical density of epidermal basal cells and fibroblasts, the length density of blood vessels, the numbers of proliferating cells and collagen deposition as well as biomechanical properties of healed wound were significantly higher in D-HAM-S group in most cases compared those of the diabetic group, or even in some cases compared to D-HAM-M group. Furthermore, in D-HAM-S group, the transcripts for genes contributing to regeneration (Tgf-β, bFgf and Vegf) upregulated more than those of D-HAM-M group, when compared to diabetic ones. Overall, the HAM-S had more impact on delayed wound healing process compared to traditional use of intact HAM. It is therefore suggested that the bioengineered three dimensional micro-porous HAM-S is more suitable for cells adhesion, penetration, and migration for contributing to wounded tissue regeneration. Keywords Diabetes mellitus · Ischemic wound · Human amniotic membrane · Three-dimensional scaffold · Regenerative medicine
Introduction Diabetes mellitus (DM) is one of the most common and challenging chronic diseases and it is estimated that the numbers of the patients will approximately reach to 700 million by 2045 [11]. One of the most important and severe common complications of DM is impairment in wound healing. More than 34 percent of diabetic patients suffer from Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00403-020-02137-3) contains supplementary material, which is available to authorized users. * Abbas Piryaei [email protected] Extended author information available on the last page of the article
diabetic foot ulcers [2], and a considerable number of them wil
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