Enhanced healing of a full-thickness wound by a thermoresponsive dressing utilized for simultaneous transfer and protect
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TISSUE ENGINEERING CONSTRUCTS AND CELL SUBSTRATES Original Research
Enhanced healing of a full-thickness wound by a thermoresponsive dressing utilized for simultaneous transfer and protection of adipose-derived mesenchymal stem cells sheet Alireza Rezapour-Lactoee1,2 Hamid Yeganeh ●
3
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Reza Gharibi4 Peiman Brouki Milan5 ●
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Received: 10 March 2020 / Accepted: 24 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract To boost the healing process in a full-thickness wound, a simple and efficient strategy based on adipose-derived mesenchymal stem cells (ADSCs) transplantation is described in this work. To increase the chance of ADSCs immobilization in the wound bed and prevent its migration, these cells are fully grown on the surface of a thermoresponsive dressing membrane under in vitro condition. Then, the cells sheet with their secreted extracellular matrix (ECM) is transferred to the damaged skin with the help of this dressing membrane. This membrane remains on wound bed and acts both as a cell sheet transfer vehicle, after external reduction of temperature, and protect wound during the healing process like a common wound dressing. The visual inspection of wounded skin (rat animal model) at selected time intervals shows a higher wound closure rate for ADSCs treated group. For this group of rats, the better quality of reconstructed tissue is approved by results of histological and immunohistochemical analysis since the higher length of the new epidermis, the higher thickness of re-epithelialization layer, a higher level of neovascularization and capillary density, and the least collagen deposition are detected in the healed tissue. Graphical Abstract
Supplementary information The online version of this article (https:// doi.org/10.1007/s10856-020-06433-2) contains supplementary material, which is available to authorized users. * Hamid Yeganeh [email protected] 1
2
Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran Department of Tissue Engineering, School of Medicine, Qom University of Medical Sciences, Qom, Iran
3
Iran Polymer and Petrochemical Institute, TehranP.O. Box:14965/ 115Iran
4
Faculty of Chemistry, Kharazmi University, Tehran, Iran
5
Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
101 Page 2 of 13
Journal of Materials Science: Materials in Medicine (2020)31:101
1 Introduction Optimum wound healing needs an organized series of cellular and molecular events, including inflammation, cell proliferation and migration, angiogenesis, and extracellular matrix (ECM) deposition and remodeling [1]. Superficial skin damages can be healed without special treatment and with minimal scarring. The healing process of such wounds can be accelerated by using proper wound dressings or other common wound treatment procedures [2–5]. However, rapid and effective intervention is needed for healing wi
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