Enhanced Oil Recovery by Potential Biosurfactant-Producing Halo-thermotolerant Bacteria Using Soil Washing and Sand-Pack
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Enhanced Oil Recovery by Potential Biosurfactant‑Producing Halo‑thermotolerant Bacteria Using Soil Washing and Sand‑Packed Glass Column Techniques Irfan Ali Phulpoto1,2 · Bakhtiar Ali Jakhrani1 · Anwar Hussain Phulpoto1 · Asif Ali Panhyar1 · Nisar Ahmed Kanhar1 · Safia Ahmed3 · Muneer Ahmed Qazi1 Received: 22 December 2019 / Accepted: 21 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Biosurfactants offer numerous advantages over the chemical surfactants, especially in energy and environment-related applications. Microbial enhanced oil recovery (MEOR) is a technique to recover oil from reservoirs by using microbes and their metabolites. In present study, total sixteen morphologically distinct bacterial strains isolated from different salty areas of the district Khairpur Mir’s, Pakistan, were investigated for their MEOR potential. Screening assays for thermotolerance and halotolerance declared 7 out of 16 (43.75%) bacterial isolates as thermotolerant (capable of growing in the temperature range 60–70 °C) and halotolerant (tolerating NaCl concentrations up to 17%, w/v). Moreover, five of them were screened as biosurfactant producers. Among, the lowest surface tension reduction was achieved with biosurfactants produced by the strains KJ2MO (27.8 mN/m) and KJ2SK (29.3 mN/m). The biosurfactant activity was found stable at temperature (100– 121 °C, 1 h) and pH (4–10). Moreover, maximum oil recovery was obtained with biosurfactant of bacterial strain KJ2MO (54.7%, 51.25%) followed by KJ2SK (44.7%, 40.5%), KJ1WB (37%, 35.5%) and KJ2MD (37.8%, 31.9%) by using either techniques, i.e., soil washing and sand-packed column, respectively. Moreover, the potent species were identified as Pseudomonas pseudoalcaligenes KJ1WB, Bacillus aerius KJ2MD, Bacillus licheniformis KJ2SK, and Bacillus subtilis KJ2MO using 16S rRNA ribo-typing. The investigated species were found to be promising biosurfactants producers having potential for enhanced oil recovery and could be used in other environmental applications like bioremediation.
Introduction Pakistan is one of the top ten countries having highest shale oil reserves comprising almost 227 billion barrels oil, but only 9.1 billion barrels oil has been reported as recoverable Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02172-3) contains supplementary material, which is available to authorized users. * Muneer Ahmed Qazi [email protected] 1
Faculty of Natural Science, Institute of Microbiology, Shah Abdul Latif University, Khairpur Mir’s Sindh 66020, Pakistan
2
College of Resources and Environment, University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China
3
Department of Microbiology, Faculty of Biological Sciences, Quaid-I-Azam University, Islamabad 45320, Pakistan
with currently available technology (USA EIA Report, 2013) [1]. The microbial enhanced oil recovery (MEOR) offers an environment-friendly, cost-effective and easy alternative to c
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