Enterocin M-Producing Enterococcus faecium CCM 8558 Demonstrating Probiotic Properties in Horses
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Enterocin M-Producing Enterococcus faecium CCM 8558 Demonstrating Probiotic Properties in Horses Andrea Lauková 1 & Eva Styková 2 & Ivana Kubašová 1 & Viola Strompfová 1 & Soňa Gancarčíková 2 & Iveta Plachá 2 & Renata Miltko 3 & Grzegorz Belzecki 3 & Igor Valocký 2 & Monika Pogány Simonová 1
# Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The effects of non-authochtonous Enterococcus faecium AL41 = CCM 8558, enterocin M-producing and probiotic strain were tested on the microbiota, phagocytic activity, hydrolytic enzymes, biochemical parameters and dry matter in horses based on its previous benefits demonstrated in other animals. E. faecium CCM 8558 sufficiently colonized the digestive tract of horses. At day 14, its counts reached 2.35 ± 0.70 CFU/g (log 10) on average. The identity of CCM 8558 was confirmed by means of PCR after its re-isolation from horse faeces. The inhibition activity of CCM 8558 was demonstrated against Gram-negative aeromonads, counts of which were significantly reduced (P < 0.001). After 14 days application of CCM 8558, a tendency towards increased phagocytic activity (PA) was measured; PA value was 73.13% ± 8.55 on average at day 0/1; at day 14, it was 75.11 ± 8.66%. Cellulolytic, xylanolytic and pectinolytic activity in horse faeces was significantly increased (P < 0.001) at day 14 (after CCM 8558 application) and amylolytic activity as well (P < 0.01) compared to day 0/1. Inulolytic activity increased with mathematical difference 1.378. Dry matter value reached 20.81 ± 2.29% on average at day 0/1; at day 14, it was 20.77 ± 2.59% (P = 0.9725). Biochemical parameters were influenced mostly in the physiological range. These results achieved after application of CCM 8558 in horses are original, giving us further opportunity to continue these studies, to measure additional parameters and to show the benefits of CCM 8558 application in horses. Keywords Enterococcus faecium . Horses . Effects . Health
Introduction The microbiology of the equine gastrointestinal tract is still poorly characterized, both in health and disease [1, 2]. However, the intestinal microbiota has enormous impact on the health and performance of horses [3]. The microbiota is unique for each horse, but certain phyla can predominate in healthy animals [4]; this indicates that the equine gut microbial community may not be as stable as that found for instance in the human gut [5]. Schoster et al. [4] reported that the
* Andrea Lauková [email protected] 1
Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01 Košice, Slovakia
2
University of Veterinary Medicine and Pharmacy, Komenského 73, 048 03 Košice, Slovakia
3
Polish Academy of Sciences, The Kielanowski Institute of Animal Physiology and Nutrition, Instytucka 3, 05 110 Jablonna, Poland
Firmicutes phylum is predominant in the faeces of horses. Among infections, the most frequently affecting the health of horse are Salmonella spp. or clostridiae [6]. Disorders caused by the B group
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