Environmental Adaptations of an Extremely Plant Beneficial Bacillus subtilis Dcl1 Identified Through the Genomic and Met
- PDF / 5,148,684 Bytes
- 16 Pages / 595.276 x 790.866 pts Page_size
- 23 Downloads / 191 Views
PLANT MICROBE INTERACTIONS
Environmental Adaptations of an Extremely Plant Beneficial Bacillus subtilis Dcl1 Identified Through the Genomic and Metabolomic Analysis Aswathy Jayakumar 1 & Indu C. Nair 2 & E. K. Radhakrishnan 1 Received: 8 April 2020 / Accepted: 16 September 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Bacterial endophytes ubiquitously colonize the internal tissues of plants and promote the plant growth through diverse mechanisms. The current study describes the mechanistic basis of plant-specific adaptations present in an extremely beneficial endophytic bacterium. Here, the endophytic Bacillus subtilis Dcl1 isolated from the dried rhizome of Curcuma longa was found to have the drought tolerance, IAA and ACC deaminase production and phosphate solubilization properties. The whole genome sequencing and annotation further showed the genome of B. subtilis Dcl1 to have the size of 4,321,654 bp. This also showed the presence of genes for IAA, H2S, acetoin, butanediol, flagella and siderophore production along with phosphate solubilization and biofilm formation for the B. subtilis Dcl1. In addition, the genes responsible for the synthesis of surfactin, iturin, fengycin, bacillibactin, bacillaene, bacilysin, chitinase, chitosanase, protease and glycoside hydrolase could also be annotated from the genome of B. subtilis Dcl1. Identification of genes for the glycine betaine, glutamate and trehalose further indicated the drought stress tolerance features of B. subtilis Dcl1. The presence of the genetic basis to produce the catalase, superoxide dismutase, peroxidases, gammaglutamyltranspeptidase, glutathione and glycolate oxidase also indicated the plant oxidative stress protective effect of B. subtilis Dcl1. Identification of these properties and the demonstration of its plant probiotic effect in Vigna unguiculata confirmed the applicability of B. subtilis Dcl1 as a biofertilizer, biocontrol and bioremediator agent to enhance the agricultural productivity. Keywords Biotic stress . Abiotic stress . LC-MS/MS . Whole genome sequencing . Bacillus subtilis
Introduction Highlights • Experimental confirmation of highly plant beneficial adaptations of endophytic Bacillus subtilis Dcl1 • Whole genome sequencing–based annotation of plant beneficial adaptations of endophytic Bacillus subtilis Dcl1 • Elucidation of plant probiotic potential of endophytic Bacillus subtilis Dcl1 Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00248-020-01605-7) contains supplementary material, which is available to authorized users. * E. K. Radhakrishnan [email protected] 1
School of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala 686 560, India
2
Department of Biotechnology, SAS SNDP Yogam College, Pathanamthitta, Kerala, India
Biotic or abiotic stress factors significantly limit the growth and development of plants and hence have a determining effect on the global food production. To manage these, various methods based
Data Loading...
