Preparation of Wetting Powder for Biocontrol Bacillus Subtilis
Bacillus subtilis is considered as one kind of beneficial strains which can effectively inhibit the pathogenic fungus of the plants and promote plant growth. B. subtilis wetting powder exhibited better prevention and cure of powdery mildew, phytophthora b
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Preparation of Wetting Powder for Biocontrol Bacillus Subtilis Fang Chen, Shangjing Guo, Haiying Shi, Deduo Han, Yuanjun Kang, Yu Zheng and Min Wang
Abstract Bacillus subtilis is considered as one kind of beneficial strains which can effectively inhibit the pathogenic fungus of the plants and promote plant growth. B. subtilis wetting powder exhibited better prevention and cure of powdery mildew, phytophthora blight, gray mold, and plant growth promoting ability. B. subtilis wetting powder was formulated using diatomite and calcium carbonate as carrier, sodium dodecyl sulfate, PVA, and CMC-Na as co-formulants, soluble starch, sucrose, and sodium glutamate as protective agent. Optimal formulation recipe and processing technology of B. subtilis wetting powder were developed. The best composition was diatomite 5 %, calcium carbonate 5 %, SDS 5 %, CMC-Na 5 %, soluble starch 0.5 %, sucrose 0.5 %, and sodium glutamate 0.5 %. Quality index analysis revealed that the wetting powder reached the biological pesticide standards. Keywords Bacillus subtilis
Wetting powder Stability Preparation
This research was supported by the Natural Science Foundation of Tianjin, China (Project No. 09JCZDJC19100). Scientific Research foundation for Doctor, Liaocheng University, China (Project No. 3010) F. Chen S. Guo H. Shi D. Han Y. Kang School of Pharmaceutical, Liaocheng University, Liaocheng, 252059 Shandong, China Y. Zheng M. Wang (&) Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Collage of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China e-mail: [email protected]
T.-C. Zhang et al. (eds.), Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), Lecture Notes in Electrical Engineering 250, DOI: 10.1007/978-3-642-37922-2_110, Ó Springer-Verlag Berlin Heidelberg 2014
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110.1 Introduction There is increasing market demand for the environmental friendly biopesticide. Antagonistic bacteria are considered as an important functional group of beneficial bacteria used for biological control as well as for promoting plant growth[1, 2]. Recently, biological control has become an important approach to suppress many plant pathogens[3–6]. Bacillus subtilis is considered to be an excellent biocontrol agent not only due to its ability on inducing plant systematic resistance, but also on producing various hydrolytic enzymes and antibiotics [7–14]. From the large amount of antimicrobials produced, lipopeptides stand among the most representative. These antifungal peptides have been proved safe to people and no pollution to environment [15, 16]. So, they have high potential for being used in biological control, food antisepsis, medicine, and so on. As B. subtilis has the characteristics of omnipresence in soils, thermal tolerance, rapid growth, and ready formation of resistant spores, it is considered to be a good biological control agent. Hot air was used in process of spray drying. Temperature was thought to be a
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