Enzymatic degradation of aqueous dextrans as affected by initial molecular weight and concentration
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Enzymatic degradation of aqueous dextrans as affected by initial molecular weight and concentration Hualei Chen1,2 · Yuanyuan Pu1,2 · Qingsong Zou1,2 · Dianzhi Hou1,2 · Shan Chen1,2 Received: 17 January 2018 / Revised: 4 July 2020 / Accepted: 17 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Dextrans with different initial molecular weights (IMW) ranging from 3.86 × 104 Da to 2.15 × 106 Da were enzymatically degraded by dextranase enzyme. The changes in weight-average molecular weight (Mw), polydispersity index (D value) and the percentage of 1 04–105 Da fragment of aqueous dextran were examined during 0–120 min enzymatic degradation. Results showed that the Mw of all dextran samples decreased with increasing enzymatic time. A significant molecular weight degradation occurred in dextran samples that had a higher IMW and at an early degradation stage. With increasing the degradation time, the percentage of the 1 04–105 Da in four dextran samples (Dex-270, Dex-1000, Dex-2000 and Dex-F) showed a remarkable growth at the beginning of the treatment (0–50 min). Experimental data indicated that all dextran samples (with different IMW and at different concentration) followed a second-order degradation kinetics. This study demonstrated a great potential for further production of low molecular weight dextrans with a more homologous molecular weight. Keywords Enzymatic degradation · Dextran · Initial molecular weight (IMW) · Kinetic
Hualei Chen and Yuanyuan Pu contributed equally to this study and they are co-first authors of the paper. * Shan Chen chen‑[email protected] 1
College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, People’s Republic of China
2
Center for Sugar Engineering and Technology Research, Guangxi University, Nanning 530004, People’s Republic of China
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Polymer Bulletin
Introduction Dextran is a homo-polysaccharide of d-glucose monomer. It is composed of α-(1 → 6) linkages in the main chain and α-(1 → 2), α-(1 → 3) and α-(1 → 4) linkages in the branch when synthesized by dextransucrase (an enzyme that catalyzes sucrose to produce dextran) [1, 2]. Dextran products with different molecular weights have potential applications in different areas. For example, high molecular weight dextran products (Mw> 106 Da) can be used as a chromatographic column filler in fine chemical industry [3]; dextrans with a molecular weight of 105
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