Epstein Barr Virus Methods and Protocols
This volume explores data from the applications of molecular biological methods and the applications of recent immunological and cytogenetic methods in Epstein-Barr Virus (EBV) that will offer readers possible new solutions to the unresolved problems in t
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Janos Minarovits Hans Helmut Niller Editors
Epstein Barr Virus Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Epstein Barr Virus Methods and Protocols
Edited by
Janos Minarovits University of Szeged, Szeged, Hungary
Hans Helmut Niller University of Regensburg, Regensburg, Germany
Editors Janos Minarovits University of Szeged Szeged, Hungary
Hans Helmut Niller University of Regensburg Regensburg, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6653-0 ISBN 978-1-4939-6655-4 (eBook) DOI 10.1007/978-1-4939-6655-4 Library of Congress Control Number: 2016957632 © Springer Science+Business Media New York 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover illustration: The picture is derived from the chapter “Functional Analysis of Exosomes Derived from EBV-Infected Cells” of this book, by Gulfaraz Khan and Pretty S. Philip. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Foreword The Epstein–Barr virus (EBV) was identified 49 years ago as the causative agent of a long time known disease, infectious mononucleosis (IM, glandular fever). The IM symptoms are not manifested by all individuals at the time of acquisition of the infection. It is highly important that the subclinical primary infection also leads to the virus carrier state for lifetime accompanied by efficient immunity. The “silent” infection is due to a particular strategy of the immune system, based on a finely tuned interaction between T and B lymphocytes. When EBV-carrying B lymphocytes acquire proliferating potential (thus malignant potential) in a well-defined differentiation windo
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