Chikungunya Virus Methods and Protocols
This volume covers multidisciplinary approaches on various aspects of Chikungunya Virus (CHIKV) research that was brought together from leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic pro
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Justin Jang Hann Chu Swee Kim Ang Editors
Chikungunya Virus Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Herts., United Kingdom
For further volumes: http://www.springer.com/series/7651
Chikungunya Virus Methods and Protocols Edited by
Justin Jang Hann Chu Department of Microbiology and Immunology, National University of Singapore, Singapore
Swee Kim Ang Department of Microbiology and Immunology, National University of Singapore, Singapore
Editors Justin Jang Hann Chu Department of Microbiology and Immunology National University of Singapore Singapore
Swee Kim Ang Department of Microbiology and Immunology National University of Singapore Singapore
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3616-8 ISBN 978-1-4939-3618-2 (eBook) DOI 10.1007/978-1-4939-3618-2 Library of Congress Control Number: 2016935402 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface Chikungunya virus (CHIKV) is a mosquito-borne, single-stranded, positive-sense RNA virus belonging to the alphavirus genus of the family Togaviridae. CHIKV infection in humans has re-emerged as a significant infectious disease which is no longer confined to developing countries in the tropics due to recent outbreaks in various geographical regions worldwide including America and Europe. Chikungunya: Methods and Protocols provides the increasing number of CHIKV researchers a useful handbook covering multidisciplinary approaches on various aspects of CHIKV research brought together by leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, and bioinformatics and
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