EST-derived microsatellites in Pseudosciaena crocea and their applicability to related species
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EST-derived microsatellites in P seudosciaena crocea and their applicability to related species YE Hua1,2 , WANG Xiaoqing1 , GAO Tianxiang3 , WANG Zhiyong2∗ 1
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
2
Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College/Fisheries Biotechnology Institute, Jimei University, Xiamen 361021, China
3
Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China
Received 8 April 2010; accepted 8 October 2010 ©The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg 2010
Abstract Simple sequence repeat (SSR) markers were obtained for the large yellow croaker Pseudosciaena crocea using 1 205 expressed sequences tags (ESTs) from the NCBI database. Primers for 48 ESTSSR loci were designed and screened with 30 P.crocea specimens captured from Guanjingyang sea area in Fujian Province of China. Sixteen of the loci were polymorphic, which were amplified with 3 to 11 alleles per locus and the mean of 6.13. The observed and expected heterozygosity per locus ranged from 0.091 to 0.844 (mean 0.544) and from 0.118 to 0.892 (mean 0.644), respectively. Polymorphic information content (PIC) ranged from 0.115 to 0.866 (mean 0.593). The results for cross-species amplification of the 16 large yellow croaker EST-SSRs on P. polyactis, C. niveatus, C. lucidus, A. argentatus and J. belengeri revealed that 14, 12, 11, 7 and 6 loci were successfully amplified with 1 to 10 alleles with an average of 4.5 per locus, respectively, which are suitable for population genetics studies of these species and useful for phylogenetic relationship analysis among these species. Overall, this study provides a set of type I markers for population genetics studies and genome mapping for large yellow croaker and its closely related species. Key words: Pseudosciaena crocea, EST-SSRs, genetic diversity, cross-species amplification
1 Introduction Large yellow croaker is one of the most economically important marine fish species in China, and mainly distributed in coastal regions of East Asia. Its wild population has severely decreased because of overfishing since the 1970s, and many commercial characteristics began to degrade with the development of culture industry, such as lower growth rate, earlier sex maturation, and lower viability. Genetic researches for the large yellow croaker have been carried out in recent years in the assistance of government (Chang et al., 2008; Li et al., 2008; Ning et al., 2007; An et al., 2005; Guo et al., 2005; Wang et al., 2002). Microsatellites of the large yellow croaker have been developed in recent years. However, there is an obvious need for more microsatellites for intensive
study on the resource management and constructing a microsatellite-based genetic map for analyzing quantitative trait loci (QTL) (Ning et al., 2007). Microsatellites consist of multiple copies of tandemly arranged simple sequence repeats (SSRs) that range in size from 1 t
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