Comparative transcriptome analysis to identify putative genes related to trichome development in Ocimum species
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ORIGINAL ARTICLE
Comparative transcriptome analysis to identify putative genes related to trichome development in Ocimum species Muktesh Chandra1 · Shiwani Kushwaha1 · Neelam S. Sangwan1,2 Received: 2 April 2020 / Accepted: 2 August 2020 © Springer Nature B.V. 2020
Abstract Genus Ocimum is known to have species possessing important therapeutic essential oil. The major phytoconstituents of essential oil in Ocimum species are phenylpropanoids and terpenoids. The essential oil is accumulated in the trichomes; the specialized structures predominantly found on leaves and other tissues. The development of trichome is integrated with development of plant and leaf and also tightly coordinated with the primary and secondary metabolic pathways producing essential oil constituents. In continuation to our studies on elucidating/understanding the mechanism of biosynthesis of essential oil pathways in Ocimum species, we have performed comparative transcriptome analysis to investigate the role of trichome-related gene expression in the regulation of biosynthetic pathways of essential oil. The essential oil biogenesis is tightly integrated with primary metabolic activities, the analysis for the expression pattern of genes related to primary metabolism and its relationship with secondary metabolism was evaluated in comparative manner. Physiological parameters in relation to primary metabolism such as photosynthetic pigment content, soluble sugar content, and invertase enzymes along with morphological parameters were analysed in O. basilicum and O. sanctum. Differential expression profiling uncovered about 8116 and 2810 differentially expressed transcripts in O. basilicum and O. sanctum, respectively. Enrichment of differentially expressed genes were analysed in relation to metabolic pathways, primary metabolism and secondary metabolism. Trichome related genes identified from the Ocimum species vis-à-vis their expression profiles suggested higher expression in O. basilicum. The findings in this study provide interesting insights into the role of trichome-related transcripts in relation to essential oil content in Ocimum species. The study is valuable as this is the first study on revealing the transcripts and their role in trichome development and essential oil biogenesis in two major species of Ocimum. Keywords Comparative transcriptomics · Ocimum · Trichome · Essential oil · Terpenoids · Tulsi · Phenylpropanoids Abbreviations Ob O. basilicum Os O. sanctum CPC Caprice ETC1 Enhancer of TRY and CPC1 ETC2 Enhancer of TRY and CPC2 Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05710-1) contains supplementary material, which is available to authorized users. * Neelam S. Sangwan [email protected] 1
Metabolic and Structural Biology Department, CSIR-Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow 226015, India
Present Address: Department of Biochemistry, Central University of Haryana, Mahendergarh, Haryana 123029, India
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