Establishment and identification of primary bovine omasal epithelial cells
- PDF / 1,205,764 Bytes
- 5 Pages / 595.276 x 790.866 pts Page_size
- 118 Downloads / 208 Views
REPORT
Establishment and identification of primary bovine omasal epithelial cells Zixuan Hu 1 & Kang Zhan 1 & Tianyu Yang 1 & Guoqi Zhao 1 & Miao Lin 1 Received: 25 February 2020 / Accepted: 7 July 2020 / Editor: Tetsuji Okamoto # The Society for In Vitro Biology 2020
Abstract The establishment and culture of bovine omasal epithelial cells (BOECs) in vitro is a valuable tool for the study of the physiological function, nutrient absorption, and transport mechanisms of the omasum in dairy cows. This paper proposes a method for the culture of primary BOECs. Trypsin digestion was used to subculture the BOECs, which were passaged for 20 generations in vitro, and showed typical epithelial-like characteristics and a cobblestone morphology. The primary BOECs had a fast growth phase (between days 4 and 5) and were validated by their slight β-galactosidase and visible cytokerat in 18 expression. In addition, RT-PCR results demonstrated that the monocarboxylate transporter 1 (MCT1), Na+/H+exchanger 1 (NHE1), and Na+/H+ exchanger 3 (NHE3) were expressed in the isolated primary BOECs. In conclusion, this primary BOEC isolation and culture model is a promising method for the study of nutrient absorption and regulation, as well as the immune regulation of epithelial cell transport in vitro.
Introduction The omasum, located on the right side of the anterior part of the abdominal cavity, is the third stomach of ruminants. Although this organ is not involved in the process of digestion, its mucosal surface forms many lobes of different sizes (Hofmann 1989). The omasum stores small feed particles and transfers them into the abomasum for enzymatic digestion (Becker et al. 1963). During the second contraction phase of the reticulum, the reticule-omasal sphincter opens for a few seconds, allowing a small volume of finely dispersed and well-fermented digesta to enter the omasum (Sjaastad et al. 2010). The inter-laminate recesses between the omasum mucosal laminae provide increased surface area for absorption (Hofmann 1989). Compared with that of other ruminants, the bovine omasum is more sophisticated, and its internal surface area is relatively large. Thus, the bovine omasum may be more suitable for secretion and absorption compared with that of other ruminants (McSweeney 1988). Nutrients, including a large amount of water, short-chain fatty acids (SCFAs), minerals, electrolytes, and amino acids, are * Miao Lin [email protected] 1
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People’s Republic of China
primarily absorbed through the omasal epithelium (Matthews and Webb Jr 1995; Rémond et al. 2000; Grünberg and Constable 2009). SCFAs absorbed by bovine omasal epithelial cells (BOECs) can provide energy and prevent the flow of a powerful buffer substance into the abomasum (Ali et al. 2006). BOECs play an essential role in nutrient absorption and regulation, and the establishment of BOECs’ cultures is useful for the study of the molecular mechanisms of omasal epithelial cell transport. According to p
Data Loading...
