Establishment of the Insulin Resistance Induced by Inflammatory Response in 3T3-L1 Preadipocytes Cell Line
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Establishment of the Insulin Resistance Induced by Inflammatory Response in 3T3-L1 Preadipocytes Cell Line Yuan Zhou,1,2 Bao-lin Liu,1,4 Kang Liu,1 Ning Tang,3 Jue Huang,2 Yuan An,1 and Lin Li1
Abstract—In the light of given recent reports, insulin resistance related to inflammation is characterized by increasing a diverse array of pro-inflammatory cytokines. In this study, hypothesizing that 3T3-L1 non-differentiated preadipocytes cell line as a cell model could be used to investigate this linkage, the aim is to determine whether the preadipocytes induced by different inflammatory responses could cause insulin resistance. This paper has determined the time and concentrationdependent effects of insulin on glucose consumption in the 3T3-L1 non-differentiated preadipocytes. Glucose consumption has also been assayed in the preadipocytes which are treated with LPS and CM originated from LPS-activated RAW264.7. Then protein level of each group has been measured by coomassie brilliant blue protein kit. Furthermore, secretion levels of IL-6 and TNF-α are measured by ELISA in the supernatant of RAW264.7 and preadipocytes. Finally, the mRNA expressions for IL-6, TNF-α and PPARγ has been assessed by RT-PCR. The results show that administration of LPS and CM both can increase releases of IL-6 and TNF-α, as well as gene expression of IL-6 mRNA; this change is accompanied with suppression of PPARγ mRNA activation in 3T3-L1 undifferentiated preadipocytes. In conclusion, our results suggest that in preadipocytes, pro-inflammatory cytokines can result in insulin resistance, and deserve further investigation to be helpful for treatment and revealing mechanisms of T2DM. KEY WORDS: 3T3-L1 preadipocyte; insulin resistance; inflammatory factor; PPARγ.
circulating concentrations of insulin insufficiently regulate glucose homeostasis. It results in loss of balance in glucose homeostasis and the development of glucose intolerance [1, 2]. The association of insulin resistance with obesity has been well recognized. Obesity is characterized by a chronic inflammatory response, abnormal elevations in cytokines production and the activation of signaling
INTRODUCTION Insulin resistance, defined as the inability of insulin to stimulate glucose uptake in insulin sensitive tissues, such as the liver, skeletal muscles, adipose tissue and also macrophages, is associated with obesity, the aging process, a sedentary lifestyle as well as a genetic predisposition. Insulin resistance occurs when normal 1
College of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210038, People’s Republic of China 2 Quality Test Centrol of Animal Product in Jiangsu, Nanjing 210036, People’s Republic of China 3 Life Science Department, Nanjing Xiaozhuang College, Nanjing 211171, People’s Republic of China 4 To whom correspondence should be addressed at College of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210038, People’s Republic of China. E-mail: [email protected]
Abbreviation: LPS, lipopolysaccharide
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