Evaluation of decellularization protocols for production of porcine small intestine submucosa for use in abdominal wall
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ORIGINAL ARTICLE
Evaluation of decellularization protocols for production of porcine small intestine submucosa for use in abdominal wall reconstruction Y. Chai1 · J. Xu2 · Y. Zhang3 · J. Zhang1 · Z. Hu1 · H. Zhou1 Received: 7 October 2018 / Accepted: 16 April 2019 © Springer-Verlag France SAS, part of Springer Nature 2019
Abstract Background Porcine-derived acellular biologic grafts are increasingly used in abdominal wall reconstruction and other soft tissue repairs. In a previous work, we have shown porcine small intestine submucosa (PSIS) exhibits clear advantages over porcine pericardium (PPC) and porcine acellular dermal matrix (PADM) in repairing full-thickness abdominal wall defects. In the present study, we aim to determine, quantify, and compare the effects of two most commonly used decellularization protocols on biomechanical and biocompatible properties of PSIS. Materials and methods After mechanical preparation, PSIS was treated with either alkaline and acid (AA) protocol or sodium dodecyl sulfate (SDS) protocol. Cellular content removal, preservation of matrix components, micro- and ultra- structures, and mechanical properties were compared. The host responses were evaluated using PSIS for repairing rat abdominal wall defects. Results and conclusion With regard to the absence of cellular contents, neatly arranged collagen fiber structures, better retention of growth factors, better mechanical strength, lower degrees of local and systemic inflammatory responses, higher degree of vascularization and tissue ingrowth, alkaline and acid protocol exhibits clear advantages over SDS protocol for the preparation of PSIS extracellular matrix. Keywords Porcine small intestine submucosa (PSIS) · Decellularization · Abdominal wall reconstruction · Biomechanical properties · Biocompatibility
Introduction Yunsheng Chai and Jian Xu contribute equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10029-019-01954-4) contains supplementary material, which is available to authorized users. * Z. Hu [email protected] * H. Zhou [email protected] 1
Division of Colorectal Surgery, Changzheng Hospital, Second Military Medical University, No. 415, Fengyang Road, Shanghai 200003, People’s Republic of China
2
Department of Surgery, Shanghai Baoshan District Combining Traditional Chinese and Western Medicine Hospital, No. 181, Youyi Road, Shanghai, People’s Republic of China
3
Department of Gastrointestinal Surgery, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, No. 528, Zhangheng Road, Shanghai, People’s Republic of China
The management of large abdominal wall defects or incisional hernias continues to be a fundamental problem in the field of hernia surgery [1]. In most cases, the use of a prosthetic graft, whether synthetic or biological, is indicated to ensure a tension-free repair for a satisfactory reconstruction [2]. Although permanent synthetic grafts have sufficient mechanical strength to withstand intra-a
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