Evaluation of Freshness of Fishes Using MWCNT/TiO 2 Nanobiocomposites Based Biosensor
- PDF / 1,660,642 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 42 Downloads / 165 Views
Evaluation of Freshness of Fishes Using MWCNT/TiO2 Nanobiocomposites Based Biosensor Jagriti Narang 1 & Nitesh Malhotra 2 & Chaitali Singhal 1 & C.S. Pundir 3
Received: 22 December 2015 / Accepted: 27 June 2016 # Springer Science+Business Media New York 2016
Abstract In present work, we have developed a highly sensitive biosensor to evaluate the freshness of fishes by determining their xanthine level. This is based on xanthine oxidase (XOx) immobilized on the nanocomposite platform of titanium dioxide nanoparticles and multi-walled carbon nanotubes (TiO2/MWCNT). The biosensor exhibited excellent performances like linear relation in the range from 0.5 to 500 μM, detection limit of 0.5 μM (S/N = 3), and a rapid response time of 30 s. The biosensor showed excellent reproducibility, high selectivity, and long-term stability (60 days). The techniques like dynamic light scattering (DLS), scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and X-ray diffraction (XRD) were used to characterize the nanocomposite modified electrode. The current biosensor is very reliable and stable for the determination of the freshness of fishes.
Keywords Freshness of fish . Gold electrode . Xanthine . Biosensor . Titanium dioxide nanoparticles . Multi-walled carbon nanotubes . Labeo
* Jagriti Narang [email protected]
1
Amity Institute of Nanotechnology, Amity University, J2 Block, 4th Floor, Noida, (UP), India
2
Holy Family Hospital, Okhla, New Delhi, India
3
Department of Biochemistry, Maharishi Dayanand University, Rohtak, Haryana, India
Introduction The aspiration to sense vital physiological analytes with elevated sensitivity has fueled nanotechnology. Moreover, amperometric detection using biosensors for sensing is widely used nowadays because of its extensive use in wide fields of application including medical diagnostics, environmental monitoring, food analysis, etc. (Shah 1996). Electrochemical biosensor kinetics including heterogeneous charge transport at the electrode/liquid interface are substantially influenced by the biosensor surface characteristics including material, shape, and geometry (Da et al. 2007; Feigel et al. 2011; Soleymani et al. 2009; Squires et al. 2008). Indeed, nanomaterials such as carbon nanotubes (CNTs), metallic nanocrystals, and silicon nanowires have significantly enhanced the analytical performance of the biosensors (Alivisatos, 2004; Balasubramanian and Burghard, 2008; Lord and Kelley, 2009; Lin et al. 2004; Chen et al. 2003; Wang 2005; Yong et al. 2009). Carbon nanotubes are nanomaterials with a huge impact on amperometric biosensors, with aptitude to bring together a number of formulations to improve current densities (Turner 2013; Singhal et al. 2016). Similarly, semiconductor nanoparticles like titanium dioxide (TiO2) have high surface area, magnetic properties, and many more properties which make them suitable as sensing platform. Thus, herein we have employed titanium dioxide nanoparticles and carboxylated multi-walled carbo
Data Loading...
