Ultrasensitive aptamer-based biosensor for acetamiprid using tetrahedral DNA nanostructures
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Ultrasensitive aptamer-based biosensor for acetamiprid using tetrahedral DNA nanostructures Y. Yao1,2,3, G. X. Wang1,2,3, X. J. Shi1,2,3, J. S. Li1,2,3, F. Z. Yang1,2,3, S. T. Cheng1,2,3, H. Zhang1,2,3, H. W. Dong1,2,3, Y. M. Guo1,2,3,*, X. Sun1,2,3,*, and Y. X. Wu4,*
1
School of Agricultural Engineering and Food Science, Shandong University of Technology, No.12 Zhangzhou Road, Zibo 255049, China 2 Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No.12 Zhangzhou Road, Zibo 255049, China 3 Zibo City Key Laboratory of Agricultural Product Safety Traceability, No.12 Zhangzhou Road, Zibo 255049, China 4 Shandong Lvdu Biotechnology Co. LTD, Binzhou 256600, China
Received: 6 February 2020
ABSTRACT
Accepted: 18 August 2020
An electrochemical tetrahedral APT biosensor (Au-TAN biosensor) was successfully constructed, which had a stable structure and excellent performance using tetrahedral DNA nanomaterials (TDN) and Au nanoparticles (AuNPs) for the detection of acetamiprid. Specifically, we designed a partial paired complementary DNA (cDNA) of acetamiprid aptamer and inserted the cDNA together with the acetamiprid aptamer into four single-stranded DNA sequences forming each side of the TDN, modifying AuNPs to increase the current signal intensity, to assemble the Au-TAN. This proposed aptasensor was compared with ssAPT aptasensor and tetrahedral APT nanostructures (TAN) aptasensor, respectively. The results showed that the performance of the designed aptasensor was better than those of the above two aptasensors. The detection limit of the aptasensor was calculated to be 0.077 pM (S/N = 3), which was far lower than that of other methods that we compared. Under optimized conditions, the proposed aptasensor showed a wide linear detection range from 0.1 pM to 10 nM. The Au-TAN biosensor opens up new ideas for the construction of aptasensors and provides a new method for detection of small molecular substances, such as pesticides residues.
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Springer Science+Business
Media, LLC, part of Springer Nature 2020
Handling Editor: Christopher Blanford.
Address correspondence to E-mail: [email protected]; [email protected]; [email protected]
https://doi.org/10.1007/s10853-020-05132-1
J Mater Sci
Introduction At present, the threat of pesticide residues to human health and environmental pollution is one of the most concerned issues of the public [1–3]. As one of the most efficient and neuro-active widespread neonicotinoid insecticides, a large amount of acetamiprid is used to control a variety of pests (Its chemical structure is shown in Fig. S1). According to some reports, acetamiprid could injury human peripheral blood lymphocytes and result in DNA damages [4–6], so its residue accumulated in agricultural products will lead to potential health risk of human beings [7–9]. Thus, the regulation and detection of acetamiprid have been of great importance. Existing methods for acetamiprid detection are largely official analytical methods, such as high-performance liquid chromatograph
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